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Functional genomics and the study of development, variation and evolution

Nature Reviews Genetics volume 2, pages 528–537 (2001)Cite this article

Key Points

  • Specification of the animal body plan and coordination of its development are accomplished through the regulation of complex networks of genes.

  • Molecular variation in gene sequences results in phenotypic variation in populations, which is the raw material on which evolutionary forces act.

  • Functional genomics technologies promise to accelerate the study of development and of evolution.

  • A synthetic approach, using the tools of molecular genetics and genomics, quantitative and population genetics, experimental evolution and comparative biology, is required to study the complex molecular basis of evolutionary change.

Abstract

It has rarely been possible to connect the developmental and evolutionary branches of genetics, particularly with regard to the precise changes in the molecular control of development that are responsible for phenotypic variation and evolution. Making such connections will require a high-resolution molecular description of the genetic networks that underlie development and an understanding of their responses to genetic and environmental variation. Functional genomics approaches to development and evolution promise to accelerate the research necessary to accomplish these goals.

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Figure 1: Microarray approaches to finding protein–DNA binding sites.

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Acknowledgements

I thank D. Houle and J. Kim who read versions of this manuscript and provided many useful discussions.

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Authors and Affiliations

  1. Department of Genetics, Yale University School of Medicine, New Haven, 06510, Connecticut, USA

    Kevin P. White

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ENCYCLOPEDIA OF LIFE SCIENCES

Evolutionary developmental biology: developmental and genetic mechanisms of evolutionary change

Evolutionary ideas: the modern synthesis

Multispot array technologies

Mass spectrometry in biology

Glossary

DRIFT

Mutation, drift and selection are the three forces of evolution on which most population genetics theory is based. Drift refers to random fluctuations in allele and, less commonly, phenotype frequencies as genes are transmitted from one generation to the next.

GENETIC NETWORK

A system of interactions among genes and their products. In creating a phenotype, genetic networks are influenced both by their constituents and the environment in which the organism develops.

MICROEVOLUTIONARY

An evolutionary pattern observed over a short period of time (up to hundreds of generations for animals or plants, and up to tens of thousands of generations for microbes).

QUANTITATIVE TRAIT

A measurable trait that depends on the cumulative action of many genes (or quantitative trait loci).

DENTICLE BELTS

Segmentally repeated features of the cuticle that are made up of tiny, rigid hair-like structures.

SEXUAL DIMORPHISM

Difference of physical form between the sexes.

TEOSINTE

The wild progenitor of maize.

FUNCTIONAL GENOMICS

The development and application of global (genome-wide or system-wide) experimental approaches to assess gene function by making use of information and reagents provided by physical mapping and sequencing of genomes.

DNA MICROARRAYS

Devices used to interrogate complex nucleic acid samples by hybridization. They make it possible to quantitate the amount of different nucleic acid molecules that are present in a sample of interest.

DIAUXIC SHIFT

The process of switching from anaerobic to aerobic respiration.

MESODERM

In animals with three tissue layers, the mesoderm is the middle layer of tissue, between the ectoderm and the endoderm. In vertebrates, it forms the skeleton, muscles, heart, spleen and many other internal organs.

BAYESIAN NETWORK

A graph with one-way edges that connect nodes. The nodes represent assertions about relationships between them. For example, node A is related to a node B by an edge that represents that A is a cause of B, with a certain probability. Networks with many interconnected nodes can be constructed.

MASS SPECTROMETRY

A technique that provides accurate information about the molecular mass and structure of complex molecules. It can identify and quantify extremely small amounts of drugs or metabolites by their mass-fragment spectrum.

FLOW CYTOMETRY

Analysis of biological material by detecting the light-absorbing or fluorescing properties of cells or subcellular fractions (that is, chromosomes) passing in a narrow stream through a laser beam.

PREZYGOTIC/POSTZYGOTIC ISOLATION

In prezygotic isolation, reproductive barriers can take the form of behavioural differences such as at the level of mate selection. In postzygotic isolation, genetic independence of different populations is maintained because of hybrid sterility and/or inviability.

ASSOCIATION STUDIES

A set of methods used to correlate polymorphisms in genotype to polymorphisms in phenotype in populations.

CLADE

A taxon or other grouping of organisms consisting of a single species and its descendents. The Drosophila melanogaster group of species numbers about 150. Within this group there is a melanogaster subgroup of nine species: D. melanogaster, D. simulans, D. sechellia, D. mauritiana, D. teissieri, D. santomea, D. yakuba, D. erecta and D. orena.

SYMPATRY

The condition in which the distributions of two species overlap and hybridization between taxa would be possible if they were not reproductively isolated by factors other than spatial separation.

GEOTAXIS

Movement in response to gravity.

LIFE HISTORY

The reproductive strategy of an organism.

VARIANCE

A statistic describing the dispersion about the mean.

STERNOPLEURAL BRISTLES

Bristles on the thorax situated between the first and second pair of legs of Drosophila.

PUNCTUATED EQUILIBRIUM

A model of evolution in which change occurs in relatively rapid bursts, followed by a longer period of stasis. Also referred to as punctuated equilibrium.

ANTAGONISTIC PLEIOTROPY

The concept that changes associated with the evolution of selected traits can cause correlated negative changes in fitness.

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White, K. Functional genomics and the study of development, variation and evolution. Nat Rev Genet 2, 528–537 (2001). https://doi.org/10.1038/35080565

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