Abstract
T-LYMPHOCYTE development takes place mainly in the thymus, where stromal cells of epithelial and haemopoietic origin are involved in inductive and selective mechanisms, which enable specific lymphocyte populations to migrate to the periphery and establish a network of immune responses. Experiments with intact animals have clarified the precursor–product relationships between thymocyte subpopulations, but the molecular mechanisms of cell interactions in the thymus are difficult to study in vivo. In an attempt to expand thymic cell populations in vivo and maintain them in vitro for such studies, we directed high levels of expression of the murine c-myc proto-oncogene in transgenic mice by inserting it into the mouse Thy-1 transcriptional unit. Such mice develop thymic tumours which contain proliferating thymocytes and, interestingly, expanded populations of epithelial cells. Both cell types can be maintained in vitro.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Williams, A. F. & Gagnon, J. Science 216, 696–703 (1982).
Leder, P. et al. Science 222, 765–771 (1983).
Corcoran, L. M., Cory, S. & Adams, J. M. Cell 40, 71–79 (1985).
Land, H., Parada, L. F. & Weinberg, R. A. Nature 304, 596–602 (1983).
Sinn, E. et al. Cell 49, 465–475 (1987).
Cole, M. D. A. Rev. Genet. 20, 361–384 (1986).
Cory, S. Adv. Cancer Res. 47, 189–234 (1986).
Kelly, K. & Siebenlist, U. A. Rev. Immun. 4, 317–338 (1986).
Stewart, T. A., Pattengale, P. K. & Leder, P. Cell 38, 627–637 (1984).
Leder, A., Pattengale, P. K., Kuo, A., Stewart, T. A. & Leder, P. Cell 45, 485–495 (1986).
Adams, J. M. et al. Nature 318, 533–538 (1985).
Langdon, W. Y., Harris, A. W., Cory, S. & Adams, J. M. Cell 47, 11–18 (1986).
Alexander, W. S., Schrader, J. W. & Adams, J. M. Molec. cell. Biol. 7, 1436–1444 (1987).
Cory, S. & Adams, J. M. A. Rev. Immun. 6, 25–48 (1988).
Hanahan, D. A. Rev. Genet. 22, 479–519 (1988).
Harris, A. W. et al. J. exp. Med. 167, 353–371 (1988).
Kollias, G. et al. Proc. natn. Acad. Sci. U.S.A. 84, 1492–1496 (1987).
Bentley, D. L. & Groudine, M. Nature 321, 702–706 (1986).
Nepvel, A. & Marcu, K. B. EMBO J. 5, 2859–2865 (1986).
Chen, S., Botteri, F., van der Putten, H., Landel, C. R. & Evans, G. Cell 51, 7–19 (1987).
Kampinga, J. et al. Thymus (in the press).
Willcox, N. et al. Am. J. Pathol. 127, 447–459 (1987).
Lampert, I. A. & Ritter, M. A. in Thymus update Vol 1 (eds M. D. Kendall & M. A. Ritter) 5–25 (Harwood Academic, New York, 1988).
Raedler, A., Arndt, R., Raedler, E., Jablonski, D. & Thiele, H.-G. Eur. J. Immun. 8, 728–730 (1978).
Ruther, U. et al. Cell 53, 847–856 (1988).
Botteri, F. M., van der Putten, H., Wong, D. F., Sauvage, C. A. & Evans, R. M. Molec. cell. Biol. 7, 3178–3184 (1987).
Lesley, J., Hyman, R. & Schulte, R. Cell Immun. 91, 397–403 (1985).
Scollay, R. & Shortman, K. Thymus 5, 245–295 (1984).
Crispe, I. N. & Bevan, M. J. J. Immun. 138, 2013–2018 (1987).
von Boehmer, H. A. Rev. Immun. 6, 309–326 (1988).
Dialynas, D. P. et al. J. Immun. 131, 2445–2451 (1983).
Scollay, R., Bartlett, P. & Shortman, K. Immun. Rev. 82, 79–103 (1984).
Bluestone, J. A., Pardoll, D. M., Sharrow, S. O. & Fowlkes B. J. Nature 326, 82–84 (1987).
Crispe, I. N., Shimonkevitz, R. P., Hussman, L. A., Kimura, J. & Allison, J. P. J. Immun. 139, 3585–3589 (1987).
Born, W., Yagüe, J., Palmer, E., Kappler, J. & Marrack, P. Proc. natn. Acad. Sci. U.S.A. 82, 2295–2929 (1985).
Melton, D. A. et al. Nucleic. Acids Res. 18, 7035–7056 (1989).
Stanton, L. W., Watt, R. & Marcu, K. B. Nature 303, 401–406 (1983).
Spanopoulou, E., Giguere, V. & Grosveld, F. Molec. cell. Biol. 8, 3847–3856 (1988).
Palmer, M., Bentley, A., Gould, K. & Townsend, A. Nucleic. Acids Res. 17, 2353 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Spanopoulou, E., Early, A., Elliott, J. et al. Complex lymphoid and epithelial thymic tumours in Thyl-myc transgenic mice. Nature 342, 185–189 (1989). https://doi.org/10.1038/342185a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/342185a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.