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Efficient lentiviral transduction of Herpesvirus saimiri immortalized T cells as a model for gene therapy in primary immunodeficiencies

Gene Therapy volume 11, pages 956–961 (2004)Cite this article

Abstract

Infection of human T lymphocytes with the Herpesvirus saimiri (HVS) yields immortalized T-cell lines (HVS-T) which retain all the phenotypical and functional characteristics of their parental cells. This represents a new experimental model for studying genetic disorders of T lymphocytes. In spite of the efforts of many laboratories, no satisfactory way has been found so far to modify HVS-T cells genetically. We have analyzed the capacity of oncoretroviral (MLV)- and lentiviral (HIV-1)-based vectors pseudotyped with vesicular stomatitis virus glycoprotein (VSVg) to transduce HVS-T cells. HIV-1-derived vectors efficiently transduced HVS-T cell lines, reaching up to 85% of cells expressing the transgene in a single round of infection. MLV-based vectors, on the other hand, were unable to transduce more than 1% of any of the HVS-T cell lines analyzed. Lentiviral-driven gene expression was maintained constant and stable in HVS-T cells for a minimum of 48 days. We also observed that although the lentiviral transduction efficiency achieved on HVS-T cells is lower than that obtained with tumor or primary endothelial cells, it is nevertheless similar to that found with activated primary T cells.

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Acknowledgements

We are especially indebted to Dr Adrian Thrasher (Institute of Child Health, University College, London, UK) for providing the HRSIN-CSGW plasmid and giving us his enthusiastic support. We are also grateful to Drs Didier Trono and Romain Zufferey (University of Geneva, Geneva, Switzerland) for supplying us with HIV packaging pCMVΔR8.91 and envelope pMD.G plasmids, to Oxford Biomedica (Oxford, UK) for the pCNCG plasmid and to Dr David A Sanders of Purdue University, West Lafayette, IN, USA, for the pLCMV plasmid. We thank Dr Jon Trout for improving the English. We acknowledge the generous continuous supply of rIL-2 (Hoffman-LaRoche, Nutley, NJ, USA) provided by the National Institutes of Health AIDS reference and reagent program (Rockville, MD, USA). This work was supported by V Framework European Union contract grant QLT-1999-01090 (to IJM and MS) and by Spanish Ministry of Health Grant FIS01/3143 to FM. MGT is a predoctoral fellow (FPU program) of the Spanish Ministry of Education and Culture.

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  1. F Martín and I J Molina: Share credit for senior authorship

Authors and Affiliations

  1. Immunology Unit, University of Granada School of Medicine, Granada, Spain

    M G Toscano & I J Molina

  2. Service of Immunology, Hospital Clinico Universitario ‘San Cecilio’, Granada, Spain

    C Frecha & F Martín

  3. Department of Cell Biology, Physiology and Immunology, University of Córdoba, Granada, Spain

    C Ortega & M Santamaría

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  1. M G Toscano
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  2. C Frecha
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Toscano, M., Frecha, C., Ortega, C. et al. Efficient lentiviral transduction of Herpesvirus saimiri immortalized T cells as a model for gene therapy in primary immunodeficiencies. Gene Ther 11, 956–961 (2004). https://doi.org/10.1038/sj.gt.3302259

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