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Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo

Gene Therapy volume 7, pages 826–829 (2000)Cite this article

Abstract

Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. In vivo electroporation has been used to enhance delivery of chemotherapeutic agents to tumors in both animal and human studies. Recently, this delivery technique has been extended to large molecules such as plasmid DNA. Here, the successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated.

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Acknowledgements

This work was supported by a University of South Florida Research and Creative Scolarship grant, the Department of Surgery, and the College of Engineering. The pulse generator used in this study was supplied by CytoPulse Sciences, Inc, Columbia, MD, USA. We thank Vania Benavides for technical assistance.

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Authors and Affiliations

  1. Center for Molecular Delivery, University of South Florida, Tampa, FL, USA

    L Heller

  2. Department of Surgery, University of South Florida College of Medicine, Tampa, FL, USA

    M J Jaroszeski, C Pottinger & R Heller

  3. Department of Pathology, University of South Florida College of Medicine, Tampa, FL, USA

    D Coppola

  4. College of Engineering, University of South Florida, Tampa, FL, USA

    R Gilbert

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  1. L Heller
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  2. M J Jaroszeski
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  4. C Pottinger
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Heller, L., Jaroszeski, M., Coppola, D. et al. Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo. Gene Ther 7, 826–829 (2000). https://doi.org/10.1038/sj.gt.3301173

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