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Neuronal inhibition by the peptide FMRFamide involves opening of S K+ channels

Nature volume 325, pages 153–156 (1987)Cite this article

Abstract

Neurotransmitters modulate the activity of ion channels through a variety of second messengers, including cyclic AMP1–4, cyclic GMP5,6 and the products of phosphatidylinositol breakdown7–9. Little is known about how different transmitters acting through different second-messenger systems interact within a cell to regulate single ion channels. We here describe the reciprocal actions of serotonin and the molluscan neuropeptide, FMRFamide10, on individual K+ channels in Aplysia sensory neurons. In these cells, serotonin causes prolonged all-or-none closure of a class of background conductance K+ channels (the S channels)11 through cAMP-dependent protein phosphorylation12–14. Using single-channel recording, we have found that FMRFamide produces two actions on the S channels; it increases the probablity of opening of the S channels via a cAMP-independent second-messenger system and it reverses the closures of S channels produced by serotonin or cAMP.

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References

  1. Siegelbaum, S. A. & Tsien, R. W. Trends Neurosci. 6, 307–313 (1983).

    Article  CAS  Google Scholar 

  2. Kennedy, M. A. Rev. Neurosci. 6, 493–525 (1983).

    Article  CAS  Google Scholar 

  3. Nestler, E. J. & Greengard, P. Nature 305, 583–588 (1983).

    Article  ADS  CAS  Google Scholar 

  4. Levitan, I. J. Membrane Biol. 87, 177–190 (1985).

    Article  CAS  Google Scholar 

  5. Dascal, N., Landau, E. M. & Lass, Y. J. Physiol., Lond. 352, 551–574 (1984).

    Article  CAS  Google Scholar 

  6. Paupardin-Tritsch, D., Hammond, C. & Gerschenfeld, H. M. Soc. Neurosci. Abstr. 11, 466 (1985).

    Google Scholar 

  7. Berridge, M. J. & Irvine, R. F. Nature 312, 315–321 (1984).

    Article  ADS  CAS  Google Scholar 

  8. Nishizuka, Y. Nature 308, 693–698 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Oron, Y., Dascal, N., Nadler, E. & Lupu, M. Nature 313, 141–143 (1985).

    Article  ADS  CAS  Google Scholar 

  10. Price, D. A. & Greenberg, M. J. Science 197, 670–671 (1977).

    Article  ADS  CAS  Google Scholar 

  11. Siegelbaum, S. A., Camardo, J. S. & Kandel, E. R. Nature 299, 4132–417 (1982).

    Article  Google Scholar 

  12. Castellucci, V. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7492–7496 (1980).

    Article  ADS  CAS  Google Scholar 

  13. Castellucci, V. F., Nairn, A. C., Greengard, P., Schwartz, J. H. & Kandel, E. R. J. Neurosci. 2, 1673–1681 (1982).

    Article  CAS  Google Scholar 

  14. Shuster, M. J., Camardo, J. S., Siegelbaum, S. A. & Kandel, E. R. Nature 313, 392–395 (1985).

    Article  ADS  CAS  Google Scholar 

  15. Klein, M. & Kandel, E. R. Proc. natn. Acad. Sci. U.S.A. 77, 6912–6916 (1980).

    Article  ADS  CAS  Google Scholar 

  16. Abrams, T. W., Castellucci, V. F., Camardo, J. S., Kandel, E. R. & Lloyd, P. E. Proc. natn. Acad. Sci. U.S.A. 81, 7956–7960 (1984).

    Article  ADS  CAS  Google Scholar 

  17. Belardetti, F., Abrams, T. W., Castellucci, V. F., Kandel, E. R. & Siegelbaum, S. A. Soc. Neurosci. Abstr. 12, 766 (1986).

    Google Scholar 

  18. Ocorr, K. A. & Byrne, J. H. Neurosci. Lett. 55, 113–118 (1985).

    Article  CAS  Google Scholar 

  19. Erxleben, C., Brezina, V. & Eckert, R. Soc. Neurosci. Abstr. 11, 170 (1985).

    Google Scholar 

  20. Brezina, V., Erxleben, C. & Eckert, R. Biophys. J. 47, 435a (1985).

    Google Scholar 

  21. Kretz, R., Shapiro, E. & Kandel, E. R. J. Neurophysiol. 55, 113–130 (1986).

    Article  CAS  Google Scholar 

  22. Ocorr, K. A., Tabata, M. & Byrne, J. H. Soc. Neurosci. Abstr. 11, 481 (1985).

    Google Scholar 

  23. Walters, E. T., Byrne, J. H., Carew, T. J. & Kandel, E. R. J. Neurophysiol. 50, 1543–1559 (1983).

    Article  CAS  Google Scholar 

  24. Rayport, S. & Schacher, S. J. Neurosci. 6, 759–763 (1985).

    Article  Google Scholar 

  25. Hodgkin, A. L. & Katz, B. J. Physiol., Lond. 208, 37–77 (1949).

    Article  Google Scholar 

  26. Patlak, J. & Horn, R. J. gen. Physiol. 79, 333–351 (1982).

    Article  CAS  Google Scholar 

  27. Brezina, V., Eckert, R. & Erxleben, C. J. Physiol., Lond. (in the press).

Download references

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Center for Neurobiology and Behaviour, Department of Pharmacology, Columbia University, College of Physicians and Surgeons, 722 West 168th Street, New York, New York, 10032, USA

    F. Belardetti & S. A. Siegelbaum

  2. Howard Hughes Medical Institute, Center for Neurobiology and Behaviour, Department of Physiology, Columbia University, College of Physicians and Surgeons, 722 West 168th Street, New York, New York, 10032, USA

    E. R. Kandel

Authors
  1. F. Belardetti
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  2. E. R. Kandel
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  3. S. A. Siegelbaum
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Belardetti, F., Kandel, E. & Siegelbaum, S. Neuronal inhibition by the peptide FMRFamide involves opening of S K+ channels. Nature 325, 153–156 (1987). https://doi.org/10.1038/325153a0

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