Abstract
Acquiring the ability to regulate morphogenesis in plants has long been an elusive goal. Previous research has established that ‘thin cell-layer’ explants from the surface of the floral branches of tobacco can be induced to form either callus, vegetative buds, flowers or roots by adjusting the pH and the ratio of auxin to cytokinin in the culture medium1–5. We now report that oligosaccharins (oligosaccharides with regulatory activity) derived from plant cell walls can, at concentrations of ∼10−8 or 10−9 M, induce tobacco expiants to form vegetative buds instead of flowers or callus, or flowers instead of vegetative buds. Other oligosaccharins can induce the expiants to form roots instead of vegetative buds. This ability of oligosaccharins to control morphogenesis suggests that these fragments are in situ regulators of morphogenesis.
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Tran Thanh Van, K., Toubart, P., Cousson, A. et al. Manipulation of the morphogenetic pathways of tobacco explants by oligosaccharins. Nature 314, 615–617 (1985). https://doi.org/10.1038/314615a0
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DOI: https://doi.org/10.1038/314615a0
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