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Appearance of new variants of membrane skeletal protein 4.1 during terminal differentiation of avian erythroid and lenticular cells

Nature volume 313, pages 238–241 (1985)Cite this article

Abstract

The erythrocyte plasma membrane is lined with a network of extrinsic proteins, mainly spectrin and actin, which constitute a reticulum tethered to the intrinsic anion transport protein of the lipid bilayer through a linker protein, ankyrin1–3. Protein 4.1 forms a stable ternary complex with spectrin and actin4–7, thereby strengthening the reticulum and anchoring it directly to the lipid bilayer8 or to another intrinsic protein, glycophorin . It has been found recently that spectrin10–14, ankyrin15,16 and protein 4.1 (refs 17–20) are not erythrocyte-specific; this has elucidated further the mechanisms of plasma membrane assembly and modelling during the differentiation of diverse tissues. We have shown previously18 that protein 4.1 in chickens is most abundant in eryth-rocytes and lens cells, but is scarce or absent from other spectrin-rich cell types. In addition, it exists as a family of related polypeptides showing differential expression in these two tissues, suggesting variant-specific functions. Here we show that the pattern of protein 4.1 variants changes during the terminal differentiation of eryth-roid and lenticular cells, with novel variants appearing in post-mitotic cells. The accumulation of these variants may lead to the final stabilization of the plasma membrane skeletons of these cells.

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References

  1. Branton, D., Cohen, C. M. & Tyler, J. Cell 24, 24–32 (1981).

    Article  CAS  Google Scholar 

  2. Bennett, V. J. Cell. Biochem. 18, 49–65 (1982).

    Article  CAS  Google Scholar 

  3. Cohen, C. M. Semin. Hemat. 20, 141–158 (1983).

    CAS  PubMed  Google Scholar 

  4. Ungewickell, E. et al. Nature 280, 811–814 (1979).

    Article  ADS  CAS  Google Scholar 

  5. Fowler, V. & Taylor, D. L. J. Cell Biol. 85, 361–376 (1980).

    Article  CAS  Google Scholar 

  6. Cohen, C. M. & Korsgren, C. Biochem. biophys. Res. Commun. 97, 1429–1435 (1980).

    Article  CAS  Google Scholar 

  7. Ohanian, V. et al. Biochemistry 23, 4416–4420 (1984).

    Article  CAS  Google Scholar 

  8. Sato, S. B. & Ohnishi, S. Eur. J. Biochem. 130, 19–25 (1983).

    Article  CAS  Google Scholar 

  9. Anderson, R. A. & Lovrien, R. E. Nature 307, 655–658 (1984).

    Article  ADS  CAS  Google Scholar 

  10. Goodman, S. R., Zagon, I. S. & Kulikowski, R. R. Proc. natn. Acad. Sci. U.S.A. 78, 7570–7574 (1981).

    Article  ADS  CAS  Google Scholar 

  11. Repasky, E. A., Granger, B. L. & Lazarides, E. Cell 29, 821–833 (1982).

    Article  CAS  Google Scholar 

  12. Glenney, J. R. et al. Cell 28, 843–854 (1982).

    Article  CAS  Google Scholar 

  13. Bennett, V., Davis, J. & Fowler, W. E. Nature 299, 126–131 (1982).

    Article  ADS  CAS  Google Scholar 

  14. Burridge, K., Kelly, T. & Mangeat, P. J. Cell Biol. 95, 478–486 (1982).

    Article  CAS  Google Scholar 

  15. Davis, J. Q. & Bennett, V. J. biol. Chem. 259, 1874–1881 (1984).

    CAS  PubMed  Google Scholar 

  16. Nelson, W. J. & Lazarides, E. Proc. natn. Acad. Sci. U.S.A. 81, 3292–3296 (1984).

    Article  ADS  CAS  Google Scholar 

  17. Cohen, C. M., Foley, S. F. & Korsgren, C. Nature 299, 648–650 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Granger, B. L. & Lazarides, E. Cell 37, 595–607 (1984).

    Article  CAS  Google Scholar 

  19. Aster, J. C. et al. Biochem. biophys. Res. Commun. 119, 726–734 (1984).

    Article  CAS  Google Scholar 

  20. Goodman, S. R. et al. Science 224, 1433–1436 (1984).

    Article  ADS  CAS  Google Scholar 

  21. Bruns, G. A. P. & Ingram, V. M. Phil. Trans. R. Soc. B266, 225–305 (1973).

    Article  CAS  Google Scholar 

  22. Weintraub, H., Campbell, G. LeM. & Holtzer, H. J. Cell Biol. 50, 652–668 (1971).

    Article  CAS  Google Scholar 

  23. Mahoney, K. A., Hyer, B. J. & Chan, L.-N. L. Devl Biol. 56, 412–416 (1977).

    Article  CAS  Google Scholar 

  24. Marks, P. A. & Rifkind, R. A. A. Rev. Biochem. 47, 419–448 (1978).

    Article  CAS  Google Scholar 

  25. Whitfield, C. F., Mylin, L. F. & Goodman, S. R. Blood 61, 500–506 (1983).

    CAS  PubMed  Google Scholar 

  26. Eisen, H., Bach, R. & Emery, R. Proc. natn. Acad. Sci. U.S.A. 74, 3898–3902 (1977).

    Article  ADS  CAS  Google Scholar 

  27. Hanna, C. & Keatts, H. C. Expl Eye Res. 5, 111–115 (1966).

    Article  Google Scholar 

  28. Piatigorsky, J. Differentiation 19, 134–153 (1981).

    Article  CAS  Google Scholar 

  29. Nelson, W. J., Granger, B. L. & Lazarides, E. J. Cell Biol. 97, 1271–1276 (1983).

    Article  CAS  Google Scholar 

  30. Granger, B. L. & Lazarides, E. Molec. cell. Biol. 4, 1943–1950 (1984).

    Article  CAS  Google Scholar 

  31. Marshall, L. M., Thureson-Klein, A. & Hunt, R. C. J. Cell Biol. 98, 2055–2063 (1984).

    Article  CAS  Google Scholar 

  32. Blikstad, I. et al. Cell 32, 1081–1091 (1983).

    Article  CAS  Google Scholar 

  33. Hamburger, V. & Hamilton, H. L. J. Morph. 88, 49–92 (1951).

    Article  CAS  Google Scholar 

  34. Staufenbiel, M. & Deppert, W. J. Cell Biol. 98, 1886–1894 (1984).

    Article  CAS  Google Scholar 

  35. Granger, B. L., Repasky, E. A. & Lazarides, E. J. Cell Biol. 92, 299–312 (1982).

    Article  CAS  Google Scholar 

  36. Ngai, J., Capetanaki, Y. G. & Lazarides, E. J. Cell Biol. 99, 306–314 (1984).

    Article  CAS  Google Scholar 

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Author notes

  1. B. L. Granger

    Present address: Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510, USA

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA

    B. L. Granger & E. Lazarides

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  1. B. L. Granger
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  2. E. Lazarides
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Granger, B., Lazarides, E. Appearance of new variants of membrane skeletal protein 4.1 during terminal differentiation of avian erythroid and lenticular cells. Nature 313, 238–241 (1985). https://doi.org/10.1038/313238a0

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