Abstract
Polyoma virus codes for three proteins involved in host cell transformation: the large, middle and small T antigens. Middle T antigen is a major transforming protein which is responsible for the induction of the phenotype of transformed cells and, without it, transformation does not occur (reviewed in refs 1–4). Middle T antigen alone can transform established cell lines5, although large, and possibly small, T antigens are also required for the full expression of the phenotype of transformed cells in media with a low concentration of serum6. A subfraction of middle T antigen is associated with a protein kinase activity which phosphorylates middle T antigen in vitro on tyrosine7–11. There is a strong correlation between the level of this kinase activity and the degree of expression of the phenotype of transformed cells7–9,12. We report here that epidermal growth factor (EGF) stimulates tyrosine phosphorylation of middle T antigen, suggesting the possibility that mitogenic growth factor(s) regulates this phosphorylation activity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ito, Y. in Viral Oncology (ed. Klein, G.) 447–480 (Raven, New York, 1980).
Eckhart, W. Adv. Cancer Res. 35, 1–25 (1981).
Griffin, B. E. & Dilworth, S. M. Adv. Cancer Res. 39, (in the press).
Benjamin, T. L. Biochim. biophys. Acta 695, 69–95 (1982).
Treisman, R., Novak, U., Favaloro, J. & Kamen, R. Nature 292, 595–600 (1981).
Rassoulzadegan, M. et al. Nature 300, 713–718 (1982).
Eckhart, W., Hutchinson, M-A, & Hunter, T. Cell 18, 925–933 (1979).
Schaffhausen, B. S., Benjamin, T. L. Cell 18, 935–946 (1979).
Smith, A. E., Smith, R., Griffin, B. E. & Fried, M. Cell 18, 915–924 (1979).
Segawa, K. & Ito, Y. Proc. natn. Acad. Sci. U.S.A. 79, 6812–6816 (1982).
Walter, G., Hutchinson, M-A., Hunter, T. & Eckhart, W. Proc. natn. Acad. Sci. U.S.A. 79, 4025–4029 (1982).
Ito, Y., Spurr, N. & Griffin, B. E. J. Virol. 35, 219–232 (1980).
Griffin, B. E. & Maddock, C. J. Virol. 31, 645–656 (1979).
Cohen, S., Carpenter, G. & King, L. Jr J. biol. Chem. 255, 4834–4842 (1980).
Ushiro, H. & Cohen, S. J. biol. Chem. 255, 8363–8365 (1980).
Carpenter, G., King, L. Jr. & Cohen, S. Nature 276, 409–410 (1978).
Ito, Y., Brocklehurst, J. R. & Dulbecco, R. Proc. natn. Acad. Sci. U.S.A. 74, 4666–4670 (1977).
Kaplan, P., Ozanne, B. Virology 123, 372–380 (1982).
Courtneidge, S. A. & Smith, A. E. Nature 303, 435–439, (1983).
Nishimura, J., Huang, J. S. & Deuel, T. F. Proc. natn. Acad. Sci. U.S.A. 79, 4303–4307 (1982).
Whittenberger, G. & Glaser, L. Proc. natn. Acad. Sci. U.S.A. 74, 2251–2255 (1977).
Ito, Y. Virology 98, 261–266 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Segawa, K., Ito, Y. Enhancement of polyoma virus middle T antigen tyrosine phosphorylation by epidermal growth factor. Nature 304, 742–744 (1983). https://doi.org/10.1038/304742a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/304742a0
This article is cited by
-
Physical and functional interaction between polyoma virus middle T antigen and insulin and IGF-I receptors is required for oncogene activation and tumour initiation
Oncogene (2009)
-
Epidermal growth factor stimulates guanine nucleotide binding activity and phosphorylation of ras oncogene proteins
Nature (1984)
-
Clinical implications of current studies in carcinogenesis
Journal of Cancer Research and Clinical Oncology (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
