Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A protein immunologically related to erythrocyte band 4.1 is found on stress fibres of non-erythroid cells

Nature volume 299, pages 648–650 (1982)Cite this article

Abstract

Erythrocyte band 4.1 is one of the three major structural proteins of the erythrocyte cytoskeleton, along with spectrin and actin1–3. When bound to the tail-end of the actin-binding protein spectrin, band 4.1 is capable of promoting the formation of stable ternary complexes with spectrin and F-actin4–7, both in solution and on the erythrocyte membrane8. Recent work has demonstrated that proteins immunologically and structurally related to spectrin, as well as ankyrin—spectrin's membrane attachment site—are present in a wide variety of non-erythroid cells9–13, although the function of these proteins in such cells is still unclear. It has been reported14 that band 4.1 is present in platelets and granulocytes. Here we show that a protein immunologically related to band 4.1 is present in a non-erythroid cell, the fibroblast, and appears by immunofluorescent labelling to be distributed uniformly along stress fibres. We suggest that by analogy with erythrocyte band 4.1 this protein may be an accessory actin-binding protein, and may serve to integrate actin filaments and actin-binding proteins.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Branton, D., Cohen, C. M. & Tyler, J. Cell 24, 24–30 (1981).

    Article  CAS  Google Scholar 

  2. Gratzer, W. B. Biochem. J. 198, 1–8 (1981).

    Article  CAS  Google Scholar 

  3. Sheetz, M. P. Biochim. biophys. Acta 557, 122–134 (1979).

    Article  CAS  Google Scholar 

  4. Tyler, J., Hargreaves, W. & Branton, D. Proc. natn. Acad. Sci. U.S.A. 76, 5192–5196 (1979).

    Article  ADS  CAS  Google Scholar 

  5. Ungewickell, E., Bennett, P. M., Calvert, R., Ohanian, V. & Gratzer, W. B. Nature 280, 811–814 (1979).

    Article  ADS  CAS  Google Scholar 

  6. Fowler, V. & Taylor, D. L. J. Cell Biol. 85, 361–376 (1980).

    Article  CAS  Google Scholar 

  7. Cohen, C. M. & Korsgren, C. Biochem. biophys. Res. Commun. 97, 1429–1435 (1980).

    Article  CAS  Google Scholar 

  8. Cohen, C. M. & Foley, S. F. Biochem. biophys. Acta 688, 691–701 (1982).

    Article  CAS  Google Scholar 

  9. Goodman, S. R., Zagan, J. S. & Kulikowski, C. R. (1981) Proc. natn. Acad. Sci. U.S.A. 78, 7570–7574.

    Article  ADS  CAS  Google Scholar 

  10. Davis, J. & Bennett, V. J. biol. Chem. 257, 5816–5820 (1982).

    CAS  PubMed  Google Scholar 

  11. Bennett, V. Nature 281, 597–599 (1979).

    Article  ADS  CAS  Google Scholar 

  12. Bennett, V. & Davis, J. Proc. natn. Acad. Sci. U.S.A. 78, 7550–7554 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Glenney, J. R., Glenney, P., Osborn, M. & Weber, K. Cell 28, 843–854 (1982).

    Article  CAS  Google Scholar 

  14. Spiegel, J. E., Beardsley, D. S. & Lux, S. E. Fedn Proc. 41, 657 (1982).

    Google Scholar 

  15. Cohen, C. M. & Foley, S. F. J. Cell Biol. 86, 694–698 (1980).

    Article  CAS  Google Scholar 

  16. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  17. Goodman, S. R., Yu, J., Whitfield, C. F., Culp, E. N. & Posnak, E. J. J. biol. Chem. 257, 4564–4569 (1982).

    CAS  PubMed  Google Scholar 

  18. Fairbanks, G., Steck, T. L. & Wallach, D. F. H. Biochemistry 13, 2606–2617 (1971).

    Article  Google Scholar 

  19. Liljas, L., Lundahl, P. & Hjerten, S. Biochim. biophys. Acta 352, 327–337 (1974).

    Article  CAS  Google Scholar 

  20. Barak, L. S., Yocum, R., Nothnagel, E. A. & Webb, W. W. Proc. natn. Acad. Sci. U.S.A. 77, 980–984 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Barak, L. S., Yocum, R. R. & Webb, W. W. J. Cell Biol. 89, 368–372 (1981).

    Article  CAS  Google Scholar 

  22. Pollard, T. D. & Weihing, R. R. CRC crit. Rev. Biochem. 2, 1–65 (1974).

    Article  CAS  Google Scholar 

  23. Herman, I. M., Crisona, N. & Pollard, T. D. J. Cell Biol. 90, 84–91 (1981).

    Article  CAS  Google Scholar 

  24. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  25. Yin, H., Albrecht, J. H. & Fattoum, A. J. Cell Biol. 91, 901–906 (1981).

    Article  CAS  Google Scholar 

  26. Fujiwara, K. & Pollard, T. D. J. Cell Biol. 71, 848–875 (1976).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Research, Division of Hematology and Oncology, St Elizabeth's Hospital, Boston, Massachusetts, 02135, USA

    Carl M. Cohen, Susan F. Foley & Catherine Korsgren

  2. Departments of Medicine and Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts, 02111, USA

    Carl M. Cohen

Authors
  1. Carl M. Cohen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susan F. Foley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Catherine Korsgren
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cohen, C., Foley, S. & Korsgren, C. A protein immunologically related to erythrocyte band 4.1 is found on stress fibres of non-erythroid cells. Nature 299, 648–650 (1982). https://doi.org/10.1038/299648a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/299648a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing