Abstract
HLA-restriction of antiviral cytotoxic T-cell responses in man, precisely analogous to the H–2-restriction first documented in murine virus infections1–3, has now been unequivocally demonstrated with influenza virus-specific4–7 and with Epstein–Barr (EB) virus-specific8–12 cytotoxic T cells reactivated in vitro. If individual effector cells of this kind recognize viral antigen in association with one particular HLA determinant, then it follows that cytotoxic T-cell preparations from HLA-hetero-zygous donors must be polyclonal with discrete component populations each functionally linked to one of the relevant HLA-A or -B antigens. Clonal expansion of individual cells from such preparations in medium containing T-cell growth factor (TCGF-medium) not only offers a means of testing this hypothesis but should also provide monospecific populations ideal for the analysis of human cytotoxic T-cell function. We describe here the establishment in vitro of EB virus-specific human cytotoxic T-cell clones each recognizing the virus-induced lymphocyte-detected membrane antigen (LYDMA) in association with one particular HLA-A or -B determinant.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Zinkernagel, R. M. & Doherty, P. C. Nature 251, 547–548 (1974).
Doherty, P. C. & Zinkernagel, R. M. J. exp. Med. 141, 502–512 (1975).
Doherty, P. C., Blanden, R. V. & Zinkernagel, R. M. Transplant Rev. 29, 89–124 (1976).
McMichael, A. J., Ting, A., Zweerink, H. J. & Askonas, B. A. Nature 270, 524–526 (1977).
Biddison, W. E. & Shaw, S. J. Immun. 122, 1705–1709 (1979).
Shaw, S. & Biddison, W. E. J. exp. Med. 149, 565–575 (1979).
McMichael, A. J., Parham, P., Brodsky, F. M. & Pilch, J. R. J. exp. Med. 152, 195s–203s (1980).
Rickinson, A. B., Wallace, L. E. & Epstein, M. A. Nature 283, 865–867 (1980).
Misko, I. S., Moss, D. J. & Pope, J. H. Proc. natn. Acad. Sci. U.S.A. 77, 4247–4250 (1980).
Moss, D. J., Wallace, L. E., Rickinson, A. B. & Epstein, M. A. Eur. J. Immun. 11, 686–693 (1981).
Fukukawa, T. et al. J. Immun. 126, 1697–1701 (1981).
Tsoukas, C. D. et al. J. Immun. 126, 1742–1746 (1981).
Rickinson, A. B. et al. Cancer Res. 41, 4216–4221 (1981).
Wallace, L. E. et al. Cell. Immun. 67, 129–140 (1982).
Jondal, M. & Targan, S. J. exp. Med. 147, 1621–1636 (1978).
Wallace, L. E., Moss, D. J., Rickinson, A. B., McMichael, A. J. & Epstein, M. A. Eur. J. Immun. 11, 694–699 (1981).
Biddison, W. E., Ward, F. E., Shearer, G. M. & Shaw, S. J. Immun. 124, 548–552 (1980).
Biddison, W. E., Shearer, G. M. & Shaw, S. J. Immun. 127, 2231–2235 (1981).
von Boehmer, H. et al. Eur. J. Immun. 9, 592–597 (1979).
Goulmy, E. et al. J. exp. Med. 152, 182s–190s (1980).
Barnstable, C. J. et al. Cell 14, 9–20 (1978).
Ledbetter, J. A. et al. J. exp. Med. 153, 310–323 (1981).
Evans, R. L. et al. Proc. natn. Acad. Sci. U.S.A. 78, 544–548 (1981).
Inouye, H., Hank, J. A., Alter, B. J. & Bach, F. J., Scand. J. Immun. 12, 149–154 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wallace, L., Rickinson, A., Rowe, M. et al. Epstein–Barr virus-specific cytotoxic T-cell clones restricted through a single HLA antigen. Nature 297, 413–415 (1982). https://doi.org/10.1038/297413a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/297413a0
This article is cited by
-
Poor outcome in post transplant lymphoproliferative disorder with pulmonary involvement after allogeneic hematopoietic SCT: 13 years' experience in a single institute
Bone Marrow Transplantation (2009)
-
T cell avidity and tumor recognition: implications and therapeutic strategies
Journal of Translational Medicine (2005)
-
Role of donor versus recipient type Epstein-Barr virus in post-transplant lymphoproliferative disorders
Springer Seminars in Immunopathology (1998)
-
Adoptive immunotherapy for Epstein-Barr virus-associated lymphoproliferative disorders complicating marrow allografts
Springer Seminars in Immunopathology (1998)
-
The human T-cell receptor
Journal of Clinical Immunology (1985)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
