Abstract
It has been proposed that the murine cell surface antigen Lyt 2 could serve to distinguish cytotoxic T lymphocytes (CTLs) and their immediate precursors, which bear Lyt 2, from helper T cells, which do not1. Later work, however, has demonstrated that Lyt 2+ cells can ‘help’ B cells mature into antibody-secreting cells, provided that the B cells differ from the allo-helper T cells at the K or D region of the H–2 major histocompatibility complex (MHC)2. Furthermore, a T-cell line has recently been described which is Lyt 2− but specifically cytotoxic for H–2I region determinants3,4. These new findings have prompted an alternative hypothesis, that Lyt 2 antigen does not discriminate between cytotoxic and non-cytotoxic (helper) T cells, but rather between I-region- and K/D-region-specific cells5. In this new view, the apparent association between presence of Lyt 2 antigen and killer T-cell function merely reflects the tendency, by no means absolute6–8, for CTLs to recognize K- or D- rather than I-region determinants9. We show here that I-region-specific murine CTLs, generated in primary in vitro culture, do in fact bear the Lyt 2 antigen, as do CTLs directed against the K region of H–2.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cantor, H. & Boyse, E. A. Cold Spring Harb. Symp. quant. Biol. 41, 23–31 (1977).
Swain, S. L. & Panfili, P. R. J. Immun. 122, 383–391 (1979).
Swain, S. L., Dennert, G., Wormsley, S. & Dutton, R. Eur. J. Immun. 11, 175–180 (1981).
Dennert, G., Swain, S. L., Waterfield, J. D., Warner, J. F. & Dutton, R. W. Eur. J. Immun. 11, 62–64 (1981).
Swain, S. L. Fedn Proc. 39, 3110–3113 (1980).
Wagner, H., Gotze, D., Ptschelinew, L. & Rollinghof, M. J. exp. Med. 142, 1477–1487 (1975).
Klein, J., Chiang, C. L. & Hauptfeld, V. J. exp. Med. 145, 450–454 (1977).
deWaal, L. P., Melief, C. J. M. & Melvold, R. W. Eur. J. Immun. 11, 258–265 (1981).
Klein, J. Springer Seminars in Immunopathology Vol. 1, 31–49 (Springer, Berlin, 1978).
Nakayama, E., Shiku, H., Stockert, E., Oettgen, H. F. & Old, L. J. Proc natn. Acad. Sci. U.S.A. 76, 1977–1981 (1979).
Shinohara, N. & Sachs, D. H. J. exp. Med. 150, 432–444 (1979).
Hollander, N., Pillemer, E. & Weissman, I. L. J. exp. Med. 152, 674–687 (1980).
Fan, J., Ahmed, A. & Bonavida, B. J. Immun. 125, 2444 (1980).
Altman, P. L. & Katz, D. D. (eds) in Inbred and Genetically Defined Strains of Laboratory Animals. Pt. 1. Mouse and Rat, 125–127 (Federation of American Societies for Experimental Biology, Bethesda, 1979).
Ledbetter, J. A. & Herzenberg, L. A. Immun Rev. 47, 63–90 (1979).
Shen, F. -W., Boyse, E. A. & Cantor, H. Immunogenetics 2, 591–595 (1975).
Gottlieb, P. D. J. exp. Med. 140, 1432–1437 (1974).
Glasebrook, A. L. et al. Immun. Rev. 54, 225–266 (1981).
Giorgi, J. V. & Warner, N. L. J. Immun. 126, 322–330 (1981).
Vidovic, D., Juretic, A., Nagy, Z. A. & Klein, J. Eur. J. Immun. 11, 499–504 (1981).
Baker, P. E., Gillis, S., Ferm, M. M. & Smith, K. A. J. Immun. 121, 2168–2173 (1978).
Wagner, H., Rollinghof, M., Pfizenmaier, K., Hardt, C. & Johnscher, G. J. Immun. 124, 1058–1067 (1980).
Okada, M. & Henney, C. S. J. Immun. 125, 300–307 (1980).
Andrus, L., Prowse, S. J. & Lafferty, K. J. Behring Institute Mitteilungen 67, 61–67 (1980).
Miller, R. A. & Stutman, O. Cell. Immun. (in the press).
Mage, M. G., McHugh, L. L. & Rothstein, T. L. J. immun. Meth. 15, 47–56 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Miller, R., Stutman, O. Monoclonal antibody to Lyt 2 antigen blocks H–2I- and H–2K- specific mouse cytotoxic T cells. Nature 296, 76–78 (1982). https://doi.org/10.1038/296076a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/296076a0
This article is cited by
-
Differences in the phenotypes of cells mediating anti‐tumour immunity at various stages of tumour progression in mice
Immunology & Cell Biology (1989)
-
Two distinct phenotypes of HLA-DR-specific cytotoxic T-cell lines
Immunogenetics (1983)
-
Alloreactive T-cell clones. Ly phenotypes predict both function and specificity for major histocompatibility complex products
Immunogenetics (1983)
-
Interactions between MHC-encoded products and cloned T-cells. I. Fine specificity of induction of proliferation and lysis
Immunogenetics (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.