Abstract
The simian virus 40 (SV40) 72-base pair (bp) tandem-repeated sequences have a crucial role as an activator element in viral gene expression. We replaced the SV40 72-bp repeat with a 72-bp repeat derived from the long terminal repeat (LTR) of cloned Moloney murine sarcoma virus (MSV) DNA. Although there is no detectable sequence homology to SV40, the MSV repeats can substitute functionally for the SV40 repeats and generate a viable virus in monkey kidney cells.
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References
Tooze, J. DNA Tumor Viruses, Molecular Biology of Tumor Viruses Pt 2 (Cold Spring Harbor Laboratory, New York, in the press).
Reed, S. I., Ferguson, J., Davis, R. W. & Stark, G. Proc. natn. Acad. Sci. U.S.A. 72, 1605–1609 (1975).
Tjian, R. Cell 13, 165–179 (1978).
Shortle, D., Margolskee, R. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 76, 6128–6131 (1979).
Shalloway, D., Kleinberger, T. & Livingston, D. M. Cell 20, 411–422 (1980).
McKay, R. & DiMaio, D. Nature 289, 810–812 (1981).
Reddy, V. B. et al. Science 200, 494–502 (1978).
Fiers, W. et al. Nature 273, 113–120 (1978).
Ghosh, P. K., Lebowitz, P., Frisque, R. J. & Gluzman, Y. Proc. natn. Acad. Sci. U.S.A. 78, 100–104 (1981).
Benoist, C. & Chambon, P. Nature 290, 304–310 (1981).
Grosschedl, R. & Birnstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).
Mathis, D. J. & Chambon, P. Nature 290, 310–315 (1981).
Van Heuverswyn, H. & Fiers, W. Eur. J. Biochem. 100, 51–60 (1979).
Waldeck, W. & Sauer, G. FEBS Lett. 71, 313–315 (1976).
Subramanian, K. N. & Shenk, T. Nucleic Acids Res. 5, 3635–3642 (1978).
Gruss, P., Dhar, R. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 78, 943–947 (1981).
Gruss, P., Dhar, R., Maizel, J. & Khoury, G. in Genes and Tumor Genes (eds Schoene, H. H. & Winnacker, E.)(Raven, New York, in the press).
Dhar, R., McClements, W. L., Enquist, L. W. G. & Vande Woude, G. Proc. natn. Acad. Sci. U.S.A. 77, 3937–3941 (1980).
Blair, D. G., McClements, W. L., Oskarsson, M. K., Fischinger, P. J. & Vande Woude, G. Proc. natn. Acad. Sci. U.S.A 77, 3504–3508 (1980).
Chang, E. H., Ellis, R. W., Scolnick, E. M. & Lowy, D. R. Science 210, 1249–1251 (1980).
Blair, D. G. et al. Science 212, 941–943 (1981).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
McClements, W. L., Enquist, L. W., Oskarsson, M., Sullivan, M. & Vande Woude, G. F. J. Virol. 35, 488–497 (1980).
Maxam, A. M. & Gilbert, W. Meth. Enym. 65, 499–599 (1980).
Seif, I., Khoury, G. & Dhar, R. Nucleic Acids Res. 6, 3387–3398 (1979).
Myers, R. M. & Tjian, R. Proc. natn. Acad. Sci. U.S.A 77, 6491–6495 (1980).
Berk, A. J. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 75, 1274–1278 (1978).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Capecchi, M. R. Cell 22, 479–488 (1980).
Ghosh, P. K. et al. Proc. natn. Acad. Sci. U.S.A. 78, 1386–1390 (1981).
Gruss, P., Efstratiadis, A., Karathanasis, S., Konig, M. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 78, 6091–6095 (1981).
Seif, I., Khoury, G. & Dhar, R. Cell 18, 963–977 (1979).
Gruss, P. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 78, 133–137 (1981).
Sutcliff, J. G. Cold Spring Harb. Symp. quant. Biol. 43, 77–90 (1979).
Morris, C. F., Hama-Inaba, H., Mace, D., Sinha, N. F. & Alberts, B. J. biol. Chem. 254, 6787–6796 (1979).
Dugaiczyk, A., Boyer, H. W. & Goodman, H. M. J. molec. Biol. 96, 174–184 (1975).
Hutchinson, K. W. & Havlorson, H. O. Gene 8, 267–278 (1980).
Clewell, D. B. J. Bact. 110, 667–676 (1972).
Wesnik, P. C., Finnegan, D. J., Donelson, J. E. & Hogness, D. S. Cell 3, 315–325 (1974).
Hirt, B. J. molec. Biol. 26, 365–369 (1967).
Penman, S. J. molec. Biol. 17, 117–130 (1966).
Gruss, P., Lai, C.-J., Dhar, R. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 76, 4317–4321 (1979).
Simmons, D. T. & Martin, M. A. Proc. natn. Acad. Sci. U.S.A. 75, 1131–1135 (1978).
Laemmli, U. Nature 227, 680–685 (1970).
Laskey, R. A. & Mills, A. D. Eur. J. Biochem. 56, 335–341 (1975).
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Levinson, B., Khoury, G., Vande Woude, G. et al. Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus. Nature 295, 568–572 (1982). https://doi.org/10.1038/295568a0
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DOI: https://doi.org/10.1038/295568a0
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