Abstract
Excitable cells use ion channels to tailor their biophysical properties to the functional demands made upon them1. During development, these demands may alter considerably, often associated with a change in the cells' complement of ion channels2,3,4. Here we present evidence for such a change in inner hair cells, the primary sensory receptors in the mammalian cochlea. In mice, responses to sound can first be recorded from the auditory nerve and observed behaviourally from 10–12 days after birth; these responses mature rapidly over the next 4 days5,6,7,8. Before this time, mouse inner hair cells have slow voltage responses and fire spontaneous and evoked action potentials. During development of auditory responsiveness a large, fast potassium conductance is expressed, greatly speeding up the membrane time constant and preventing action potentials. This change in potassium channel expression turns the inner hair cell from a regenerative, spiking pacemaker into a high-frequency signal transducer.
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Acknowledgements
This work was begun at the University of Sussex and was supported by MRC programme grants to I. J. Russell, G. P. Richardson and C.J.K., and a DFG postdoctoral grant to A.R. We thank R. A. Eatock for providing facilities for additional experiments at Baylor College of Medicine, and E.Glowatzki, M. C. Holley, G. P. Richardson and I. J. Russell for commenting on an early version of the manuscript. C.J.K. is a Royal Society University Research Fellow.
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Table of all cells contributing to Figure 2. (DOC 35 kb)
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Acutely isolated organ of Corti of a P18 mouse (basal coil, 540-720 degrees from helicotrema). (GIF 286 kb)
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Acutely isolated organ of Corti of a P18 mouse (basal coil, 540-720 degrees from helicotrema). (TXT 0 kb)
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Kros, C., Ruppersberg, J. & Rüsch, A. Expression of a potassium current in inner hair cells during development of hearing in mice. Nature 394, 281–284 (1998). https://doi.org/10.1038/28401
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DOI: https://doi.org/10.1038/28401
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