Abstract
BECAUSE of its physiological importance, allosteric properties and ready availability, D-glyceraldehyde-3-phosphate dehydrogenase (G3PDH) is one of the most thoroughly studied of the dehydrogenases. We report here a study of the ligand binding properties of G3PDH. We observed that when active site carboxymethylated G3PDH was irradiated with near ultraviolet light in the presence of NAD+, a new fluorophore was formed.
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HO, YS., TSOU, CL. Formation of a new fluorophore on irradiation of carboxymethylated D-glyceraldehyde-3-phosphate dehydrogenase. Nature 277, 245–246 (1979). https://doi.org/10.1038/277245a0
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DOI: https://doi.org/10.1038/277245a0
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