Abstract
THE differentiation of B and T lymphocytes by scanning electron microscopy and, in live suspensions, by light microscopy has led to controversy about their different surface characteristics1,2. They can be demonstrated serologically by the presence or absence of specific antigen markers in various animal species, including man. B cells can be recognised among mouse lymphocytes by surface immunoglobulin staining under fluorescent microscopy4. The surface morphology of lymphocytes has been distinguished by light microscopy using Nomarski differential interference5. We have now used the Hoffman modulation contrast microscopy system6,7 and confirmed that B and T lymphocytes have different surface characteristics. This technique makes it possible to observe both live cells and air-dried smears, and we have demonstrated the feasibility of restoring the distinct surface characteristics in blood smears.
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PADNOS, M. Differentiation of B and T mouse lymphocytes in cell suspension and smears. Nature 259, 218–220 (1976). https://doi.org/10.1038/259218a0
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DOI: https://doi.org/10.1038/259218a0
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