Abstract
THE growth and differentiation of the mammary gland is controlled by the synergistic action of insulin, prolactin and ovarian or corticosteroid hormones1–4. The ovarian steroid induced alveolar development of the virgin mammary gland in vivo is mediated by activation of nucleic acid and protein synthesis and mitosis5–9. On the other hand, numerous studies using organ cultures, mostly of fragments of mid-pregnant mammary gland, emphasised the role of insulin as the mitogenic hormone in mammary gland development10–12. This variable nature of responsiveness of the mammary tissue to hormones in vivo and in organ culture has been discussed in recent reports13,14. The proliferative mammary gland of pregnancy, mostly used in these organ culture studies10–12, should contain a large proportion of cells in the division cycle at the time of excision; we considered that hormonal regulation of the fate of these dividing cells during incubation in vitro may influence the interpretation of the results obtained from the organ culture studies. In order to test this possibility, mid-pregnant mammary cells of Balb/c mice were labelled for 1 h with 3H-thymidine (3H-dT) in vivo, and the transit patterns of the labelled cells reaching mitosis were determined at different times in organ culture.
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References
Nandi, S., J. natn. Cancer Inst., 21, 1039 (1958).
Nandi, S., and Bern, H. A., Gen. Comp. Endocr., 1, 195 (1961).
Rivera, E. M., in Methods in Mammalian Embryology (edit. by Daniel, J. A.), 442 (Freeman, 1971).
Banerjee, M. R., and Rogers, R. M., J. Endocr., 49, 39 (1970).
Banerjee, M. R., Banerjee, D. N., and Mehta, R. G., J. natn. Cancer Inst., (in the press).
Banerjee, M. R., Mehta, R. G., and Wagner, J. W., J. natn. Cancer Inst., 50, 339 (1973).
Banerjee, D. N., Banerjee, M. R., and Wagner, J. W., J. Endocr., 51, 259 (1971).
Ichinose, R. R., and Nandi, S., J. Endocr., 35, 331 (1966).
Mehta, R. G., and Banerjee, M. R., J. cell Biol., 55, 174a (1972).
Stockdale, F. E., and Topper, Y. J., Proc. natn. Acad. Sci. U.S.A., 56, 1283 (1966).
Topper, Y. J., and Oka, T., in Symposium on Effects of Drugs on Cellular Control Mechanisms (edit. by Rabin, B., and Freedman, R. B.), 131 (Macmillan, London, 1971).
Turkington, E. W., in Biochemical Actions of Hormones (edit. by Litwack, G.), 2, 55 (Academic Press, New York, 1972).
Mayne, R., and Barry, J. M., J. Endocr., 46, 61 (1970).
Rivera, E. M., in Problems in Reproduction (edit. by Josimovich, J. D.) (Wiley, N.Y.M.2, 1972, in the press).
Bresciani, F., Expl Cell Res., 38, 13 (1965).
Banerjee, M. R., and Walker, R. J., J. cell Comp. Physiol., 69, 133 (1967).
Friedberg, S. H., Oka, T., and Topper, Y. J., Proc. natn. Acad. Sci. U.S.A., 67, 1493 (1970).
Stockdale, F. E., Juergens, W. G., and Topper, Y. J., Devl Biol., 13, 266 (1966).
Oka, T., and Topper, Y. J., Proc. natn. Acad. Sci. U.S.A., 69, 1693 (1972).
Turkington, R. W., and Kadohama, N., Acta endocr., Copenh., 5, 346 (1972).
Tata, J. R., in Biochemical Actions of Hormones (edit. by Litwack, G.), 1, 89 (Academic Press, New York, 1970).
Puca, G. A., and Bresciani, F., Endocrinology, 85, 1 (1969).
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MUKHERJEE, A., WASHBURN, L. & BANERJEE, M. Role of Insulin as a ‘Permissive’ Hormone in Mammary Gland Development. Nature 246, 159–160 (1973). https://doi.org/10.1038/246159a0
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DOI: https://doi.org/10.1038/246159a0
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