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Animal Models

A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma

Leukemia volume 22, pages 170–178 (2008)Cite this article

Abstract

A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma was established by subcutaneously implanting the sample taken from the patient with secondary extranodal nasal type NK/T-cell lymphoma of the stomach into the right axillary region of a BALB/c (nu/nu) nude mouse. This model had been successfully transplanted in vivo for thirty-two generations with a stable growth cycle. The survival rates of both resuscitation and transplantation were 100%. Histologically, the tumor cells were medium to large size and arranged in sheets, with a little mesenchyma, and disseminated almost in all passages of the lymphoma-bearing nude mice. Immunologically, the tumor cells were positive for CD56, cytoplasmic CD3, granzyme B or TIA-1 and LMP1, sometimes for CD8 but negative for surface CD3, CD7, CD20 and CD1a. EBER1/2 was found. No T-cell receptor γ gene rearrangement was detected in the transplanted tumors. Furthermore, both human sequencing-tagged sites SY14 and Y chromosome were detected by PCR or fluorescent in situ hybridization, respectively, in the transplanted tumor. The transplanted tumor in this novel nude mice model maintained the essential features of human extranodal nasal type NK/T-cell lymphoma, and it would be an ideal tool in vivo for further research of the tumor.

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References

  1. Jaffe ES, Harris NL, Stein H, Vardiman JW . World Health Organization Classification of Tumors. Pathology and Genetics of Tumors of Haematopoietic and Lymphoid Tissues. IARC press: Lyon, 2001, pp 204–247.

    Google Scholar 

  2. Jaffe ES, Chen JKC, Su IJ, Glauco F, Shigeo M, Feller AC et al. Report of the workshop on nasal and related extranodal angiocentric T/NK cell lymphomas: definition, differential diagnosis, epidemiology. Am J Surg Pathol 1996; 20: 103–111.

    Article  CAS  Google Scholar 

  3. Jaffe ES, Krenacs L, Kumar S, Kingma DW, Raffeld M . Extranodal peripheral T cell and NK cell neoplasms. Am J Clin Pathol 1999; 111 (Suppl 1): S46–S55.

    CAS  Google Scholar 

  4. Chiang AKS, Chan ACL, Srivastava G, Ho FCS . Nasal T/natural killer(NK)-cell lymphomas derived from Epstein-Barr virus-infected cytotoxic lymphocytes of both NK- and T-cell lineage. Int J Cancer 1997; 73: 332–338.

    Article  CAS  Google Scholar 

  5. Weiping L, Gandi L, Yonghui L, Chuanyu L . Nasal NK/T cell lymphoma—Report of 15 years research. Lin Chuang Yu Shi Yan Bing Li Xue Za zhi 2000; 16: 89–92.

    Google Scholar 

  6. Qionglan T, Weiping L, Gandi L, Chuanyu L, Yonghui L, Huan X et al. Follow-up observation of 140 upper-respiration tract NK/T cell lymphomas. Zhong Hua Er Bi Yan Hou Ke Xue Za zhi 2002; 37: 393–394.

    Google Scholar 

  7. Kagami Y, Nakamara S, Suzuki R, IiDA S, Yatabe Y, Okada Y et al. Establishment of an IL-2-dependent cell line derived from ‘nasal-type’ NK/T-cell lymphoma of CD2+, SCD3-, CD3ɛ+, CD56+ phenotype and associated with the Epstein-Barr virus. Br J Haematol 1998; 103: 669–677.

    Article  CAS  Google Scholar 

  8. Tsuchiyama J, Yoshino T, Mori M, Kondoh E, Oka T, Akagi T et al. Characterization of a novel human natural killer cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection. Blood 1998; 92: 1374–1383.

    CAS  Google Scholar 

  9. Nagata H, Konno A, Kimura N, Zhang Y, Kimura M, Demachi A et al. Characterization of a novel human natural killer (NK)-cell and γδ T cell lines established from primary lesions of nasal-cell lymphomas associated with the Epstein-Barr virus. Blood 2001; 97: 708–713.

    Article  CAS  Google Scholar 

  10. Lin YW, Nichols RA, Letterio JJ, Aplan PD . Notch1 mutations are important for leukemic transformation in murine models of precursor-T leukemia/lymphoma. Blood 2006; 107: 2540–2543.

    Article  CAS  Google Scholar 

  11. Rashidi B, Jiang P, Jiang P . A nude mouse model of massive liver and lymph node metastasis of human colon cancer. Anticancer Res 2000; 20: 715–722.

    CAS  Google Scholar 

  12. Wistuba II, Behrens C, Milchgrub S, Syed S, Ahmadian M, Virmani AK et al. Comparison of features of human lung cancer cell lines and their corresponding tumors. Clin Cancer Res 1999; 5: 991–1000.

    CAS  Google Scholar 

  13. Denyssevych T, Lestou VS, Knesevich S, Robichaud M, Salski C, Tan R et al. Establishment and comprehensive analysis of a new human transformed follicular lymphoma B cell line, Tat-1. Leukemia 2002; 16: 276–283.

    Article  CAS  Google Scholar 

  14. Wistuba II, Behrens C, Milchgrub S, Syed S, Ahmadian M, Virmani AK et al. Comparison of features of human breast cancer cell lines and their corresponding tumors. Clin Cancer Res 1998; 4: 2931–2932.

    CAS  Google Scholar 

  15. Yagita M, Huang CL, Umehara H, Matsuo Y, Tabata R, Miyake M et al. A novel natural killer cell line (KHYG-1) from a patient with aggressive natural killer cell leukemia carrying a p53 point mutation. Leukemia 2000; 14: 922–930.

    Article  CAS  Google Scholar 

  16. Lovejoy EA, Clarke AR, Harrison DJ . Animal models and the molecular pathology of cancer. J. Pathol 1997; 181: 130–135.

    Article  CAS  Google Scholar 

  17. Kubota T . Metastatic models of human cancer xenografted in the nude mouse: the importance of orthotropic transplantation. J Cell Biochem 1994; 56: 4–8.

    Article  CAS  Google Scholar 

  18. Bernard N, Devevey L, Jacquemont C, Chrétien P, Helissey P, Guillosson JJ et al. A new model of pre-B acute lymphoblastic leukemia chemically induced in rats. Exp Hematol 2005; 33: 1130–1139.

    Article  CAS  Google Scholar 

  19. Schlade-Bartusiak K, Sasiadek MM, Bar JK, Urbschat S, Blin N, Montenarh M et al. Cytogenetic and molecular cytogenetic characterization of the stable ovarian carcinoma cell line (OvBH-1). Cancer Genet Cytogenet 2006; 164: 10–15.

    Article  CAS  Google Scholar 

  20. Urzua U, Frankenberger C, Gangi L, Mayer S, Burkett S, Munroe DJ . Microarray comparative genomic hybridization profile of a murine model for epithelial ovarian cancer reveals genomic imbalances resembling human ovarian carcinomas. Tumour Biol 2005; 26: 236–244.

    Article  CAS  Google Scholar 

  21. Williams SV, Adams J, Coulter J, Summersgill BM, Shipley J, Knowles MA . Assessment by M-FISH of karyotypic complexity and cytogenetic evolution in bladder cancer in vitro. Genes Chromosomes Cancer 2005; 43: 315–328.

    Article  CAS  Google Scholar 

  22. Han JY, Goh RY, Seo SY, Hwang TH, Kwon HC, Kim SH et al. Cotransplantation of cord blood hematopoietic stem cells and culture-expanded and GM-CSF-/SCF-transfected mesenchymal stem cells in SCID mice. J Korean Med Sci 2007; 22: 242–247.

    Article  Google Scholar 

  23. Abraham EJ, Kodama S, Lin JC, Ubeda M, Faustman DL, Habener JF . Human pancreatic islet-derived progenitor cell engraftment in immunocompetent mice. Am J Pathol 2004; 164: 817–830.

    Article  Google Scholar 

  24. Yoon YS, Wecker A, Heyd L, Park JS, Tkebuchava T, Kusano K et al. Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction. J Clin Invest 2005; 115: 326–338.

    Article  CAS  Google Scholar 

  25. Allen RJ, Smith SD, Moldwin RL, Lu MM, Giordano L, Vignon C et al. Establishment and characterization of a megakaryoblast cell line with amplification of MLL. Leukemia 1998; 12: 1119–1127.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We wish to thank Professor Norio Shimizu (Department of Virology, Tokyo Medical and Dental University, Japan) and Dr Yu Zhang (First Department of Internal Medicine, Tokyo Medical University, Japan) for providing us with human extranodal nasal type NK/T-cell lymphoma cell line, SNK6. This work was supported by National Natural Science Foundation of China (30470747 and 30570769).

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Authors and Affiliations

  1. Department of Pathology, Institute of Pathology, West China Hospital, Sichuan University, Chengdu, China

    S Zhao, Q L Tang, M X He, F Yang, W Y Zhang, W Jiang, X L Wang, G D Li & W P Liu

  2. Genetic Laboratory, West China Second University Hospital, Sichuan University, Chengdu, China

    H Wang

  3. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China

    X M Mo

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  1. S Zhao
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  2. Q L Tang
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Correspondence to W P Liu.

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Zhao, S., Tang, Q., He, M. et al. A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma. Leukemia 22, 170–178 (2008). https://doi.org/10.1038/sj.leu.2404945

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