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‘Reply to Pitiot et al.’

Leukemia volume 21, pages 2055–2056 (2007)Cite this article

We thank Pitiot et al.1 for clarifying that nucleolar positivity in their patient's leukaemic cells could be due to the wild-type NPM protein. We fully agree with this interpretation, which is also in keeping with the results of previous immunohistochemical studies.2

Since the C-terminus-truncated NPM mutant retains the amino-terminal domain that is responsible for forming heterodimers with the wild-type NPM protein,3 we expect aberrant accumulation of C-terminus-truncated NPM in leukaemic cell cytoplasm4, 5 to result in cytoplasmic dislocation of at least a fraction of wild-type NPM protein. Proof could be obtained by staining the patient's leukaemic cells with a monoclonal antibody (Invitrogen, clone FC-61991) that recognizes the wild-type but not mutated NPM.3

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Figure 1

References

  1. Pitiot AS, Santamaria I, Garcia-Suarez O, Centeno I, Astudillo A, Rayon C et al. Reply to ‘Aberrant cytoplasmic expression of C-terminus truncated NPM leukaemic mutant is dictated by tryptophans loss and a new NES motif’ by Falini et al. Leukemia 2007, in press.

  2. Falini B, Martelli MP, Bolli N, Bonasso R, Ghia E, Pallotta MT et al. Immunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia. Blood 2006; 108: 1999–2005.

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  3. Falini B, Nicoletti I, Bolli N, Martelli MP, Liso A, Gorello P et al. Translocations and mutations involving the nucleophosmin (NPM1) gene in lymphomas and leukaemias. Haematologica 2007; 92: 519–532.

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  4. Pitiot AS, Santamaria I, Garcia-Suarez O, Centeno I, Astudillo A, Rayon C et al. A new type of NPM1 gene mutation in AML leading to a C-terminal truncated protein. Leukemia 2007; [e-pub ahead of print, 5 April 2007] (doi:10.1038/sj.leu.2404679).

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  5. Albiero E, Madeo D, Bolli N, Giaretta I, Bona ED, Martelli MF et al. Identification and functional characterization of a cytoplasmic nucleophosmin leukaemic mutant generated by a novel exon-11 NPM1 mutation. Leukemia 2007; 21: 1099–1103.

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  6. Falini B, Albiero E, Bolli N, De Marco MF, Madeo D, Martelli M et al. Aberrant cytoplasmic expression of C-terminus truncated NPM leukaemic mutant is dictated by tryptophans loss and a new NES motif. Leukemia 2007, in press.

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Authors and Affiliations

  1. Institute of Haematology, University of Perugia, Perugia, Italy

    B Falini, N Bolli, M F De Marco & M Martelli

  2. Department of Cell Therapy and Haematology, San Bortolo Hospital, Vicenza, Italy

    E Albiero, D Madeo & F Rodeghiero

  3. Institute of Internal Medicine, University of Perugia, Perugia, Italy

    I Nicoletti

Authors
  1. B Falini
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  2. E Albiero
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  3. N Bolli
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  4. M F De Marco
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  5. D Madeo
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  6. M Martelli
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  7. I Nicoletti
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  8. F Rodeghiero
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Correspondence to B Falini.

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Falini, B., Albiero, E., Bolli, N. et al. ‘Reply to Pitiot et al.’. Leukemia 21, 2055–2056 (2007). https://doi.org/10.1038/sj.leu.2404841

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