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Differential expression of NF-κB pathway genes among peripheral T-cell lymphomas

Leukemia volume 19, pages 2254–2263 (2005)Cite this article

Abstract

Nuclear factor kappa B (NF-κB) is one important pathway in T-cell proliferation and survival. In a previously reported microarrray study, we found NF-κB pathway genes differentially expressed between peripheral (PTCL) and lymphoblastic lymphomas. Here, we investigated the expression of NF-κB pathway genes using cDNA microarrays in a group of 62 PTCL and in reactive lymph nodes. We found two different subgroups of PTCL based on the expression of NF-κB pathway genes. One-third of PTCL showed clearly reduced expression of NF-κB genes, while the other group was characterized by high expression of these genes. This distinction was found among all T-cell lymphoma categories analyzed (PTCL unspecified, angioimmunoblastic, cutaneous and natural killer/T lymphomas) with the exception of anaplastic lymphomas (ALCL), which were characterized by reduced NF-κB expression in anaplastic cells. Quantitative RT-PCR and immunohistochemical analysis of NF-κB-p65 protein confirmed these differences among PTCL subgroups. Importantly, we found that differentiation between NF-κB-positive and -negative PTCL could be of clinical interest. The expression profile associated to reduced expression of NF-κB genes was significantly associated with shorter survival of patients and seems to be an independent prognostic factor in a multivariate analysis.

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Acknowledgements

This work was supported by grants from the Comunidad Autonoma de Madrid CAM 08.6/0005.1/2001 and Fondo Investigaciones Sanitarias FIS G03/179. MC and EH are fellows of the Fondo de Investigación Sanitaria (FIS). We thank all the people involved in the Spanish Cooperative Group for the Study of T-cell lymphomas for their collaboration. We thank Victoria Fernandez and Alicia Barroso for their excellent technical assistance, the CNIO Histology and Immunohistochemistry Unit, and Amanda Wren for kindly reviewing the manuscript. We also thank the CNIO Tumour Bank for help in providing tumour samples.

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Author notes

  1. Spanish Cooperative Group for the study of T-cell lymphomas: B Martinez-Delgado, M Cuadros, J Benitez, Centro Nacional de Investigaciones Oncológicas (Madrid); C Rivas, Fundacion Jimenez Diaz (Madrid); J Alves Ferreira, Hospital La Paz (Madrid); J Menarguez, Hospital Gregorio Marañon (Madrid); M Mollejo, Hospital Virgen de la Salud (Toledo); C Bellas, Hospital Puerta de Hierro (Madrid); E Campo, Hospital Clinico (Barcelona); A Sáez, F Jiménez, Hospital Clinico San Cecilio (Granada); S Nieto, A Acevedo, Hospital La Princesa (Madrid); JL Rodríguez Peralto, Hospital 12 Octubre (Madrid); M García Cosío, C Montalbán, Hospital Ramón y Cajal (Madrid); J Briones, I Espinosa, Hospital San Pau (Barcelona); T Alvaro, Hospital Verge de la Cinta (Tortosa); P Domínguez, Hospital de Alcorcón (Madrid); J Gonzalez Carralero, Hospital Xeral-Cies (Vigo); J Forteza, Hospital Clínico Universitario DE Santiago de Compostelal; T Contra, Hospital Niño Jesús (Madrid)

Authors and Affiliations

  1. Human Genetics Department, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

    B Martínez-Delgado, M Cuadros, E Honrado & J Benítez

  2. Pathology and Internal Medicine Departments, Fundación Jimenez Diaz, Madrid, Spain

    A Ruiz de la Parte, J M Castrillo & C Rivas

  3. Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

    G Roncador

  4. Pathology Department, Hospital La Paz, Madrid, Spain

    J Alves

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Correspondence to J Benítez.

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Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu).

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Martínez-Delgado, B., Cuadros, M., Honrado, E. et al. Differential expression of NF-κB pathway genes among peripheral T-cell lymphomas. Leukemia 19, 2254–2263 (2005). https://doi.org/10.1038/sj.leu.2403960

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