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Evaluation of real-time quantitative PCR machines for the monitoring of fusion gene transcripts using the Europe against cancer protocol

Leukemia volume 19, pages 305–307 (2005)Cite this article

TO THE EDITOR

Real-time quantitative PCR (RQ-PCR) is rapidly becoming a standard practice, which provides a sensitive and reproducible measurement of relative gene expression by comparing one or more genes of interest to a known internal control for normalization. RQ-PCR is widely used for detection of minimal residual disease in hematologic malignancies.1, 2 In onco-hematology, the typical example of the use of RQ-PCR is represented by the dosage of BCR–ABL transcript in positive BCR–ABL leukaemias, which makes it possible to appreciate the effectiveness of the innovating treatments such as Imatinib mesylate (STI571) (inhibiting the activity tyrosine kinase of ABL).3 A collaborative action within the Europe Against Cancer (EAC) program defined and standardized RQ-PCR protocols suitable for measuring the level of fusion gene (FG) transcripts in leukemia.4, 5 This standardized protocol developed on ABI 7700® (Applied Biosystems) was used in the present study to evaluate and compare the technical outcomes of six RQ-PCR machines in the context of fusion gene transcript monitoring.

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Acknowledgements

This work was supported by a grant from the French Ministry of Employment and Solidarity for the: ‘Innovative and Expensive Diagnostic and Therapeutic Tools Programme.’ We would like to thank the laboratories which participated in the first GBMHM workshop especially Becker M, Cornillet-Lefebvre P, Fontenay M, Gachard N, Leotard B, Letestu R, Lippert E, Marzac C, Mozziconacci MJ and Sorel N. We would also like to thank all the companies who participated in this study by loaning their machines.

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Authors and Affiliations

  1. Laboratoire de Biochimie et Biologie Moléculaire Hôpital Nord, AP-HM, Marseille, France

    M Silvy & J Gabert

  2. ERT-MEIDIA, Faculté de Médecine Nord, Marseille, France

    M Silvy & J Gabert

  3. DIM AP-HM, Marseille, France

    J Mancini & X Thirion

  4. Institut d'hématologie, Hôpital Saint Louis, Paris, France

    F Sigaux

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  1. M Silvy
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  2. J Mancini
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  3. X Thirion
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  4. F Sigaux
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  5. J Gabert
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Correspondence to J Gabert.

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Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu).

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Silvy, M., Mancini, J., Thirion, X. et al. Evaluation of real-time quantitative PCR machines for the monitoring of fusion gene transcripts using the Europe against cancer protocol. Leukemia 19, 305–307 (2005). https://doi.org/10.1038/sj.leu.2403590

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  • DOI: https://doi.org/10.1038/sj.leu.2403590

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