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Apparent absence of BRCA2 protein in a proportion of acute myeloid leukemia cell lines

Leukemia volume 18, pages 1918–1920 (2004)Cite this article

TO THE EDITOR

Fanconi anemia (FA) is an autosomal recessive disease characterized by a variety of congenital anomalies, hematological problems and cancer susceptibility.1 At the cellular level, FA cells are hypersensitive to DNA crosslinking agents such as mitomycin C (MMC) and diepoxybutane, a feature that has been utilized to confirm the FA diagnosis. FA is genetically heterogeneous and comprises at least 11 complementation groups (A, B, C, D1, D2, E, F, G, I, J, L),2, 3 each of which is considered to represent one disease gene. Remarkably, patients of complementation group D1 turned out to possess biallelic mutations in the breast/ovarian cancer predisposition gene, BRCA2.4

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References

  1. Joenje H, Patel KJ . The emerging genetic and molecular basis of Fanconi anaemia. Nat Rev Genet 2001; 2: 446–457.

    Article  CAS  Google Scholar 

  2. Levitus M, Rooimans MA, Steltenpool J, Cool NF, Oostra AB, Mathew CG et al. Heterogeneity in Fanconi anemia: evidence for two new genetic subtypes. Blood 2003; 103: 2498–2503.

    Article  Google Scholar 

  3. Meetei AR, de Winter JP, Medhurst AL, Wallisch M, Waisfisz Q, van de Vrugt HJ et al. A novel ubiquitin ligase is deficient in Fanconi anemia. Nat Genet 2003; 35: 165–170.

    Article  CAS  Google Scholar 

  4. Howlett NG, Taniguchi T, Olson S, Cox B, Waisfisz Q, De Die-Smulders C et al. Biallelic inactivation of BRCA2 in Fanconi anemia. Science 2002; 297: 606–609.

    Article  CAS  Google Scholar 

  5. Xie Y, de Winter JP, Waisfisz Q, Nieuwint AW, Scheper RJ, Arwert F et al. Aberrant Fanconi anaemia protein profiles in acute myeloid leukaemia cells. Br J Haematol 2000; 111: 1057–1064.

    Article  CAS  Google Scholar 

  6. Tischkowitz M, Ameziane N, Waisfisz Q, De Winter JP, Harris R, Taniguchi T et al. Bi-allelic silencing of the Fanconi anaemia gene FANCF in acute myeloid leukaemia. Br J Haematol 2003; 123: 469–471.

    Article  CAS  Google Scholar 

  7. Ikeda H, Matsushita M, Waisfisz Q, Kinoshita A, Oostra AB, Nieuwint AW et al. Genetic reversion in an acute myelogenous leukemia cell line from a Fanconi anemia patient with biallelic mutations in BRCA2. Cancer Res 2003; 63: 2688–2694.

    CAS  PubMed  Google Scholar 

  8. Aunoble B, Bernard-Gallon D, Bignon YJ . Regulation of BRCA1 and BRCA2 transcript in response to cisplatin, adriamycin, taxol and ionising radiation is correlated to p53 functional status in ovarian cancer cell lines. Oncol Rep 2001; 8: 663–668.

    CAS  PubMed  Google Scholar 

  9. Su LK, Wang SC, Qi Y, Luo W, Hung MC, Lin SH . Characterization of BRCA2: temperature sensitivity of detection and cell-cycle regulated expression. Oncogene 1998; 17: 2377–2381.

    Article  CAS  Google Scholar 

  10. Alter BP . Cancer in Fanconi anemia, 1927-2001. Cancer 2003; 97: 425–440.

    Article  Google Scholar 

Download references

Acknowledgements

We greatly acknowledge the support from the Dutch Royal Academy of Sciences (KNAW) and the Ministry of Science and Technology of China (Grant number 00CDP029). This work was financially supported by the Dutch Cancer Society and the Netherlands Organization for Health Research and Development. We thank Ron van Schooten for MLPA analysis and Roland Kanaar for providing the anti-RAD51 antibody.

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Authors and Affiliations

  1. Department of Clinical Genetics and Human Genetics, VU University Medical Center, Amsterdam, The Netherlands

    F Chen, A L Medhurst, J P de Winter, Q Waisfisz, M A Rooimans, A B Oostra, G Pals, F Arwert & H Joenje

  2. Department of Internal Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China

    F Chen, K J Zhang & B Xia

  3. Cancer-Immunogenetics Laboratory, University of Manchester, Manchester, UK

    S Meyer

  4. Department of Pediatrics, VU University Medical Center, Amsterdam, The Netherlands

    C M Zwaan

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  1. F Chen
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  2. A L Medhurst
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Correspondence to H Joenje.

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Chen, F., Medhurst, A., de Winter, J. et al. Apparent absence of BRCA2 protein in a proportion of acute myeloid leukemia cell lines. Leukemia 18, 1918–1920 (2004). https://doi.org/10.1038/sj.leu.2403473

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