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Age-related changes of P-glycoprotein-mediated rhodamine 123 efflux in normal human bone marrow hematopoietic stem cells

Leukemia volume 17, pages 816–818 (2003)Cite this article

TO THE EDITOR

The MDR1 gene encodes the P-glycoprotein (P-gp), an energy-dependent transmembrane efflux pump for a variety of unrelated, hydrophobic amphipathic drugs.1 It reduces cytoplasmic drug levels and its cytotoxic effects, thus leading to multidrug resistance (MDR). P-gp is expressed by different normal cells, including the hematopoietic tissue. In hematopoietic stem cells, P-gp is physiologically related to cell protection against toxic substances and metabolites.1,2 Moreover, it has the ability to cause the MDR phenotype in leukemic cells.3 P-gp expression is a poor prognostic factor in acute leukemia, particularly acute myeloid leukemia (AML), and is mainly associated with a more immature phenotype and the expression of the CD34 antigen.4 The prevalence of P-gp expression in AML progressively increases with age,5 being very high among elderly patients, but the reason for this finding is not completely understood.6 The aim of the present study was to evaluate age-related changes of P-gp function in normal human bone marrow stem cells.

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Acknowledgements

This work was supported by FAPESP (Grant 98/14247-6) and CNPq (Grant 520.786/96-3). RT Calado was a recipient of FAPESP Grant 00/13885-0.

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Authors and Affiliations

  1. Department of Clinical Medicine, Center for Cell-based Therapy, Ribeirão Preto, SP, Brazil

    R T Calado, C G Machado, A B Garcia & R P Falcão

  2. Department of Surgery and Anatomy, University of São Paulo at Ribeirão Preto Medical School, Ribeirão Preto, SP, Brazil

    J J Carneiro

  3. Blood Center of Pernambuco, Recife, PE, Brazil

    C G Machado

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  1. R T Calado
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Calado, R., Machado, C., Carneiro, J. et al. Age-related changes of P-glycoprotein-mediated rhodamine 123 efflux in normal human bone marrow hematopoietic stem cells. Leukemia 17, 816–818 (2003). https://doi.org/10.1038/sj.leu.2402853

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