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G-CSF regulation of SRE-binding proteins in myeloid cells

Leukemia volume 16, pages 2332–2333 (2002)Cite this article

The hematopoietic growth factor granulocyte colony-stimulating factor (G-CSF) regulates myeloid progenitor cell proliferation, differentiation and survival. G-CSF mediates its effects through interaction with the G-CSF receptor (G-CSFR) which leads to activation of intracellular signaling pathways, including the Janus tyrosine kinase/signal transducer and activator of transcription (JAK/STAT), ras/mitogen-activated protein kinase (MAPK), phosphatidylinositol (3)-kinase (PI3K)/AKT, and protein kinase C pathways (PKC).1,2,3 The nuclear targets of most of these pathways have not been elucidated.

We previously demonstrated that the serum response element-1 (SRE-1) between nucleotides −418 and −391 of the egr-1 promoter is responsive to G-CSF in the myeloid leukemia cell line, NFS60.4 The SRE-1 contains a central serum response element (SRE) and two adjacent ETS protein-binding sites (EBS). In electromobility shift assays, the serum response factor (SRF) binds constitutively to the CArG box of the SRE whereas the ETS protein Fli-1 binds the 5′ Ets binding site (EBS). Furthermore, the SRE and EBSs of SRE-1 are each required for maximal transcriptional activation of a reporter construct containing a single copy of SRE-1 in response to G-CSF.

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Acknowledgements

This work was supported by the National Institutes of Health CA68221 (KMS and PM-G), American Cancer Society RPG-99-081-01-LBC, and California Cancer Research Program 99-00557V-10021 (KMS). KMS is also a Scholar of the Leukemia and Lymphoma Society of America and recipient of awards from CaPCURE And UC BioSTAR/Celgene-Signal Pharmaceuticals 00-10107.

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Authors and Affiliations

  1. Department of Pediatrics, Mattel Children's Hospital at UCLA, Gwynne-Hazen Cherry Memorial Laboratories, and the UCLA Jonsson Comprehensive Cancer Center, Los Angeles, CA, USA

    P Mora-Garcia, R Pan & KM Sakamoto

  2. Department of Cellular and Molecular Pathology, UCLA School of Medicine, Los Angeles, CA, USA

    KM Sakamoto

  3. Division of Biology, California Institute of Technology, Pasadena, CA, USA

    KM Sakamoto

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  1. P Mora-Garcia
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  2. R Pan
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Mora-Garcia, P., Pan, R. & Sakamoto, K. G-CSF regulation of SRE-binding proteins in myeloid cells. Leukemia 16, 2332–2333 (2002). https://doi.org/10.1038/sj.leu.2402686

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