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The novel t(11;12;18)(q21;q13;q21) represents a variant translocation of the t(11;18)(q21;q21) associated with MALT-type lymphoma

Leukemia volume 16, pages 1863–1864 (2002)Cite this article

The chromosomal translocation t(11;18)(q21;q21) characterizes about one-third of extranodal marginal zone B cell lymphomas of the MALT-type.1,2,3 We and others have recently shown that this translocation leads to a fusion of the apoptosis inhibitor gene API2 on chromosome 11 and the novel MLT/MALT1 gene, which encodes a human paracaspase, on chromosome 18.4 The pathogenetic relevant event involves the derivative chromosome 11 and leads to linkage of the three baculovirus IAP repeat (BIR) domains present in the N terminus of API2 and a variable part of MLT/MALT1, which always contains the caspase p20-like domain.1,3,4,5The chimeric protein effectively activates NF-κB, a potential pro-survival signal in B cells.6

Interphase FISH was performed according to standard methods using cytospin preparations of cells isolated from the snap-frozen lung tumor. Five hundred interphase cells were analyzed for each probe. For the detection of the 5′API2-3′MLT/MALT1 fusion resulting from the t(11;18)(q21;q21), P1 artificial chromosome (PAC) clones 59N7 and 116G16 were used as previously described.2 PAC 59N7 contains genomic sequences derived immediately downstream of the MLT/MALT1 gene. PAC clone 166G16 spans approximately 100 kb and contains the complete API2 gene. The cut-off level for diagnosing the t(11;18) was 4.4%.2 The FISH analysis showed fusion signals in 15% of analyzed cells, which indicates the presence of a 5′API2-3′MLT/MALT1 fusion.

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Acknowledgements

This work was supported by grant 70–2644Di2 from the Deutsche Krebshilfe/Dr Mildred Scheel Stiftung für Krebsforschung. M Baens and P Marynen were supported by a grant of the ‘Belgische Federatie tegen Kanker’.

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Authors and Affiliations

  1. Department of Oncology and Hematology, University Hospital Eppendorf, Hamburg, Germany

    J Dierlamm, EM Murga Penas, K Hinz, G Schilling & DK Hossfeld

  2. Department of Internal Medicine, Kochi Medical School, Kochi, Japan

    M Daibata, H Tagushi & I Miyoshi

  3. Center for Human Genetics and Flanders Interuniversity Institute for Biotechnology, University of Leuven, Belgium

    M Baens, J Cools, L Michaux & P Marynen

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Dierlamm, J., Murga Penas, E., Daibata, M. et al. The novel t(11;12;18)(q21;q13;q21) represents a variant translocation of the t(11;18)(q21;q21) associated with MALT-type lymphoma. Leukemia 16, 1863–1864 (2002). https://doi.org/10.1038/sj.leu.2402683

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