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Characterization of the t(4;14)(p16.3;q32) in the KMS-18 multiple myeloma cell line

Leukemia volume 15, pages 864–865 (2001)Cite this article

Chromosomal translocations involving the immunoglobulin heavy chain (IGH) locus at chromosome 14q32 represent a frequent event in multiple myeloma (MM). We and others have identified a new recurring t(4;14)(p16.3;q32) chromosomal translocation, which involves the most telomeric region of chromosome 4 in which the FGFR3 gene (a member of the tyrosine-kinase receptor family FGFR1–4) and the novel gene WHSC1/MMSET (a putative transcription factor) are located.123

Otsuki et al4 have recently characterized the KMS-18 cell line established from an MM patient, which shows several numerical and structural chromosomal abnormalities including a putative, non-karyotypically detectable t(4;14)(p16.3;q32) that may involve the FGFR3 locus. In order to characterize this lesion further, we performed a FISH analysis as we previously described.5 Using painting probes specific for chromosomes 4 and 14, we detected two apparently normal chromosomes 4 (data not shown) and two unidentified chromosomes bearing sequences from chromosome 14 (Figure 1a1). One of the structurally altered chromosomes was recognized by the probes specific for the Cα region of the IGH locus and the FGFR3 locus (cosmid clone 7.1), which colocalized in its telomeric region (Figure 1a2). In addition, the clone 7.1 recognized one of the two apparently normal chromosomes 4 (Figure 1a2). Finally, co-hybridization with the JH region and the cosmid A1 specific for the 3′ region of the MMSET locus revealed associated signals on the putative der(4) (Figure 1a3). These data indicate that the t(4;14)(p16.3;q32) in the KMS-18 cell line leads to a complex chromosomal rearrangement, and that the 4p16.3 breakpoint is located between MMSET and FGFR3 loci.

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Figure 2
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Acknowledgements

This work was supported by grants from the Associazione Italiana Ricerca sul Cancro (AIRC) (to AN), the Ministero della Sanità to Ospedale Maggiore IRCCS and MURST 1999 No. 9906038391-010.

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  1. Department of Medical Science, Hematology Service, Università di Milano Ospedale Maggiore, IRCCS, Milan, Italy

    D Ronchetti, S Bogni, P Finelli, L Lombardi, AT Maiolo & A Neri

  2. Department of Hygiene, Kawasaki Medical School, Okayama, Japan

    T Otsuki

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  1. D Ronchetti
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Ronchetti, D., Bogni, S., Finelli, P. et al. Characterization of the t(4;14)(p16.3;q32) in the KMS-18 multiple myeloma cell line. Leukemia 15, 864–865 (2001). https://doi.org/10.1038/sj.leu.2402097

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