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CD13/aminopeptidase N involvement in dendritic cell maturation

Leukemia volume 15, pages 190–191 (2001)Cite this article

Recently it has been shown that CD13/aminopeptidase N (APN) is involved in the development of dendritic cells (DC) and macrophages from cord blood CD34+ cells.1 More specifically, CD13 expression was positively correlated with cell proliferation, and inhibition of CD13/APN resulted in apoptosis.

Previously, we have reported expression of CD13/APN by DC in the human lung,2 as well as by various populations of mouse DC.34 Given the implication of CD13/APN activity in a variety of cellular functions and the prominent expression by DC, we approached the question of whether CD13/APN is functionally involved with DC maturation. To test this, we cultured purified human monocytes in culture fluid with granulocyte–monocyte colony-stimulating factor (GM-CSF) and interleukin (IL)-4 for 5 days, resulting in the development of CD1a-positive immature DC.5 These cultures were performed in the absence or presence of CD13/APN inhibitors: the potent and CD13/APN-specific inhibitor leuhistin and the less potent inhibitor bestatin (both 10−3 M). As an additional control, monocytes were cultured for 5 days in culture fluid without GM-CSF and IL-4. Subsequently, the cells were immunophenotyped by flow cytometry and viability of the cells was determined by propidium iodide exclusion.

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  1. Department of Immunology, Erasmus University, Rotterdam, The Netherlands

    VHJ van der Velden, PJM Leenen & HA Drexhage

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  1. VHJ van der Velden
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  2. PJM Leenen
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  3. HA Drexhage
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van der Velden, V., Leenen, P. & Drexhage, H. CD13/aminopeptidase N involvement in dendritic cell maturation. Leukemia 15, 190–191 (2001). https://doi.org/10.1038/sj.leu.2401952

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