Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Manuscript
  • Published:

Cytogenetics

Monosomy 20 as a pointer to dicentric (9;20) in acute lymphoblastic leukemia

Leukemia volume 14, pages 241–246 (2000)Cite this article

Abstract

Twenty new cases of acute lymphoblastic leukemia (ALL) with the dicentric chromosome dic(9;20)(p1113;q11) are presented. This chromosomal abnormality is difficult to identify from G-banding alone. It masquerades as monosomy 20 and is only accurately identified by fluorescence in situ hybridization (FISH). Monosomy 20 was found in 59/2790 patients with successful karyotypes entered to the Leukaemia Research Fund/UK Cancer Cytogenetics Group Karyotype Database in ALL (LRF/UKCCG Karyotype Database). FISH revealed dic(9;20) in 20/25 cases with available material. Extra copies of chromosome 21 were found in 8 of the 20 cases. Patients were 14 females and six males, aged 1–32 years (median 4 years), with leukocyte counts of 2–536 (median 23) × 109/l and immunophenotypes of common or pre-B ALL (17 cases), T-ALL (one case) or unknown (two cases). Four patients relapsed at 2, 22, 28 and 47 months and two died at 49 and 63 months (median follow-up 37 months). FISH studies on the remaining five patients showed one with monosomy 20 and four with other rearrangements of the chromosome. This study has increased the number of reported cases of dic(9;20) from 17 to 37. It has identified dic(9;20) in one case of T-ALL and shows an association of this translocation with trisomy 21.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  1. Rieder H, Schnittger S, Bodenstein H, Schwonzen M, Wormann B, Ludwig WD, Hoelzer D, Fonatsch C . dic(9;20): a new recurrent chromosome abnormality in adult acute lymphoblastic leukemia Genes Chromosom Cancer 1995 13: 54–61

    Article  CAS  Google Scholar 

  2. Slater R, Smit E, Kroes W, Jotterand Bellomo M, Muhlematter D, Harbott J, Behrendt H, Hahlen K, Veerman AJP, Hagemeijer A . A non-random chromosome abnormality found in precusor-B lineage acute lymphoblastic leukaemia: dic(9;20)(p1?3;q11) Leukemia 1995 9: 1613–1619

    CAS  PubMed  Google Scholar 

  3. Heerema NA, Maben KD, Bernstein J, Breitfeld PP, Neiman RS, Vance GH . Dicentric (9;20)(p11;q11) indentified by fluorescence in situ hybridization in four pediatric acute lymphoblastic leukemia patients Cancer Genet Cytogenet 1996 92: 111–115

    Article  CAS  Google Scholar 

  4. ISCN . 1995 An International System for Human Cytogenetic Nomenclature S Karger: Basel 1995 pp 1–114.

  5. Griffin TC, Tomlinson GE, Raimondi SC, Sandoval C, Timmons CF, Rosenfield C, Carroll AJ, Schneider NR . Childhood acute lymphoblastic leukemia with both t(1;19) and t(9;22) Leukemia 1992 6: 535–540

    CAS  PubMed  Google Scholar 

  6. Diaz MO, Rubin CM, Harden A, Ziemin S, Larson RA, Le Beau MM, Rowley JD . Deletions of interferon genes in acute lymphoblastic leukemia New Engl J Med 1990 322: 77–82

    Article  CAS  Google Scholar 

  7. Middleton PG, Prince RA, Williamson IK, Taylor PR, Reid MM, Jackson GH, Katz F, Chessells JM, Proctor SJ . Alpha interferon gene deletions in adults, children and infants with acute lymphoblastic leukemia Leukemia 1991 5: 680–682

    CAS  PubMed  Google Scholar 

  8. Ogawa S, Hirano N, Sato N, Takahashi T, Hangaishi A, Tanaka K, Tanaka T, Mitani K, Yazaki Y et al. Homozygous loss of the cyclin-dependent kinase 4-inhibitor (p16) gene in human leukemias Blood 1994 84: 2431–2435

    CAS  PubMed  Google Scholar 

  9. Okuda T, Shurtleff SA, Valentine MB, Raimondi SC, Head DR, Behm F, Liu Q, Pui CH, Sherr CJ et al. Frequent deletion of p16INK4a/MTS1 and p15INK4b/MTS2 in pediatric acute lymphoblastic leukemia Blood 1995 85: 2321–2330

    CAS  Google Scholar 

  10. Stranks G, Height SE, Mitchell P, Jadayel D, Yuille MA, De Lord C, Clutterbuck RD, Treleaven JG, Powles RL, Nacheva E, Oscier D, Karpas A, Lenoir G, Smith SD, Millar J, Catovsky D, Dyer MSS . Deletions and rearrangement of CDKN2 in lymphoid malignancy Blood 1995 85: 893–901

    CAS  Google Scholar 

  11. Heyman M, Rasool O, Borgonovo Brandter L, Liu Y, Grander D, Soderhall S, Gustavsson G, Einhorn S . Prognostic importance of p15INK4B and p16INK4 gene inactivation in childhood acute lymphocytic leukemia J Clin Oncol 1996 14: 1512–1520

    Article  CAS  Google Scholar 

  12. Fizzotti M, Cimino G, Pisegna S, Alimena G, Quartarone C, Mandelli F, Pelicci PG, Lo Coco F . Detection of homozygous deletions of the cyclin-dependent kinase 4 inhibitor (p16) gene in acute lymphoblastic leukemia and association with adverse prognostic features Blood 1995 85: 2685–2690

    CAS  PubMed  Google Scholar 

  13. Takeuchi S, Bartram CR, Seriu T, Miller CW, Tobler A, Janssen JW, Reiter A, Ludwig WD, Zimmermann M, Schwaller J, Lee E, Miyoshi I, Koeffler HP . Analysis of a family of cyclin-dependent kinase inhibitors: p15/MTS2/INK4B, p16/MTS1/INK4A, and p18 genes in acute lymphoblastic leukemia of childhood Blood 1995 86: 7557–7560

    Google Scholar 

  14. UKCCG . United Kingdom Cancer Cytogenetics Group. Translocations involving 9p and/or 12p in acute lymphoblastic leukemia Genes Chromosom Cancer 1992 5: 255–259

    Article  Google Scholar 

  15. Behrendt H, Charrin C, Gibbons B, Harrison CJ, Hawkins JM, Heerema NA, Horschler-Botel B, Huret JL, Lai JL, Lampert F, Nelken B, Perot C, Ritterbach J, Schlegelberger B, Secker-Walker LM, Slater S, Slovak ML, Tanzer J, Van Den Akker J . Dicentric (9;12) in acute lymphocytic leukemia and other hematological malignancies: report from a dic(9;12) study group Leukemia 1995 9: 102–106

    CAS  PubMed  Google Scholar 

  16. Mahmoud H, Carroll AJ, Behm F, Raimondi SC, Schuster J, Borowitz M, Land V, Pullen DJ, Vietti TJ, Crist W . The non-random dic(9;12) translocation in acute lymphoblastic leukemia is associated with B-progenitor phenotype and an excellent prognosis Leukemia 1992 6: 703–707

    CAS  PubMed  Google Scholar 

  17. Raimondi SC, Privitera E, Williams DL, Look AT, Behm F, Rivera GK, Crist WM, Pui CH . New recurring chromosomal translocations in childhood acute lymphoblastic leukemia Blood 1991 77: 2016–2022

    CAS  PubMed  Google Scholar 

  18. Berger R, Busson-Le Coniat M . Centric and pericentric chromosome rearrangements in hematopoietic malignancies Leukemia 1999 13: 671–678

    Article  CAS  Google Scholar 

  19. Silengo M, Vassallo E, Barisone E, Miniero R, Madon E . Monosomy 20 in childhood acute lymphoblastic leukemia Cancer Genet Cytogenet 1992 59: 177–179

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank the members of the following UKCCG laboratories for providing patient samples: Bristol; Cambridge; Glasgow; Leeds; Liverpool; Manchester; Newcastle; Oxford; Salisbury; Sheffield; University College Hospital, London: the Medical Research Council Leukaemia Working Parties, in particular the co-ordinators of the MRC Infant protocol (J Chessells), UKALL XI (I Hann and F Hill), ALL 97 (J Lilleyman, S Kinsey and C Mitchell), UKALL XII (I Franklin): R Clack and J Burrett, Clinical Trial Service Unit, Oxford: the Leukaemia Research Fund for financial support: and Professor Mel Greaves for providing the RT-PCR results to exclude ETV6/AML1 fusion in some cases.

Author information

Authors and Affiliations

  1. Department of Haematology, Leukaemia Research Fund/United Kingdom Cancer Cytogenetics Group Karyotype Database in Acute Lymphoblastic Leukaemia, Royal Free and University College Medical School, London, UK

    R Clark, S-A Byatt, CF Bennett, M Bra ma, M Martineau, AV Moorman, K Roberts, LM Secker-Walker & CJ Harrison

  2. Clinical Trial Service Unit and Epidemiological Studies Unit, Radcliffe Infirmary, Oxford, UK

    S Richards

  3. Academic Unit of Paediatric Oncology, Christie Hospital (NHS Trust), Manchester, UK

    OB Eden

  4. Department of Haematology, University College Hospital, London, UK

    AH Goldstone

Authors
  1. R Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S-A Byatt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. CF Bennett
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M Bra ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M Martineau
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. AV Moorman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. LM Secker-Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. S Richards
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. OB Eden
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. AH Goldstone
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. CJ Harrison
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Clark, R., Byatt, SA., Bennett, C. et al. Monosomy 20 as a pointer to dicentric (9;20) in acute lymphoblastic leukemia. Leukemia 14, 241–246 (2000). https://doi.org/10.1038/sj.leu.2401654

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.leu.2401654

Keywords

This article is cited by

Search

Advanced search

Quick links