Abstract
IMMUNOADSORBANTS are used chiefly for the isolation of antibodies against protein antigens. Glutaraldehyde has already been successfully used as a cross-linking agent to convert carboxypeptidase A1, trypsin2 and protein antigens3 into insoluble derivatives retaining their specific biological properties. Few attempts have been made to prepare immunoadsorbents from substances other than proteins4,5. In so far as lipopolysaccharides have not been insolubilized, we have attempted their cross-linkage to develop a cross-linking procedure, using glutaraldehyde.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Quicho, F. A., and Richards, F. M., Proc. US Nat. Aead. Sci., 52, 833 (1964).
Habeeb, A. F., Arch. Biochem. Biophys., 119, 264 (1967).
Avrameas, S., and Ternynck, T., Immunochemistry, 6, 53 (1969).
Kaplan, M. E., and Kabat, E. A., J. Exp. Med., 123, 1061 (1966).
Radvany, A., Neale, N. A., and Nowotny, A., Ann. NY Acad. Sci., 133, 763 (1966).
Edwards, R. P., and Ewing, W. H., in Identification of Enterobacteriaceae (Burgess, New York, 1964).
Westphal, O., Lüderitz, O., and Bister, F., Z. Naturforsch., 76b, 148 (1952).
Kochwa, S., and Rosenfield, R. E., J. Immunol., 92, 682 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ESKENAZY, M. Isolation of Anti-polysaccharide Antibodies with Glutaraldehyde Cross-linked Lipopolysaccharides. Nature 226, 855–856 (1970). https://doi.org/10.1038/226855a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/226855a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.