Abstract
MAMMALIAN RNA polymerase1 has been obtained in a soluble form from tumours2,3, chick embryo4, rat testis5 and rat liver nuclei6,7. The extraction procedures used involved the disruption of cell nuclei by sonication or exposure either to a hypotonic solution or alkaline pH. As a consequence, extracts were grossly contaminated with nuclear DNA and other cellular components and the yields of the enzyme were usually very low. We describe here a procedure for the rapid extraction from liver nuclei of fairly large yields of soluble RNA polymerase with fairly high specific activity.
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LIAO, S., SAGHER, D. & FANG, SM. Isolation of Chromatin-free RNA Polymerase from Mammalian Cell Nuclei. Nature 220, 1336–1337 (1968). https://doi.org/10.1038/2201336a0
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DOI: https://doi.org/10.1038/2201336a0
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