Abstract
THE use of tritiated thymidine as a specific precursor of DNA is well established in the study of DNA synthesis and the kinetics of cell population. The usefulness of the method depends on the conservation of labelled DNA and on the rapid catabolism of non-utilized nucleoside. During experiments in which mammalian nuclei were treated with deoxyribonuclease (DNase I) I found that labelled nucleic acid was more labile to the enzyme than DNA which carried no isotope—an observation which may have a bearing on the use of radioactive precursors.
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ROBINS, A. Relative Susceptibilities of Isotopically Labelled and Unlabelled Nuclei to Deoxyribonuclease I. Nature 215, 1291–1292 (1967). https://doi.org/10.1038/2151291a0
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DOI: https://doi.org/10.1038/2151291a0
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