Abstract
ETHIDIUM bromide (EB) (2,7 di-amino-phenyl phenanthridine 10-ethyl bromhydrate) is an antimicrobial drug which, like proflavine (an acridine), forms reversible complexes with nucleic acids1,2. In vitro, it inhibits DNA-polymerase and, at a lesser rate, DNA-dependent RNA-polymerase2. In the same way, EB and related phenanthridine derivatives inhibit the synthesis of nucleic acids in bacteria3 and Ehrlich ascites cells4; they are also trypanocidal5. Moreover, the photosensitization of arboviruses6, the inhibition of their development7 and that of a Pseudomonas pyocyanea phage8 by these compounds have been reported. Although the responses of several virus-infected systems to the action of acridines have been given in detail9,10, the comparable interactions with EB have not yet been so clearly described. The present paper details the action of EB on herpes virus and its growth in baboon kidney cells.
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VILAGINES, R., ATANASIU, P. Action of Ethidium Bromide on Growth of Herpes Virus in Cell Cultures. Nature 215, 87–88 (1967). https://doi.org/10.1038/215087a0
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DOI: https://doi.org/10.1038/215087a0
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