Abstract
PENICILLIN acylase (E.G. 3.5.1.11) hydrolyses penicillins to give 6-aminopenicillanic acid (6APA). It has been found together with the side-chain organic acid in various microorganisms1–7. Several strains of E. coli have been found with a significant intracellular splitting activity on benzylpenicillin8–10. The characteristics of the enzyme responsible for this splitting activity have been investgated both in collected intact cells2,9,11–13 and in crude enzyme preparations14–17.
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NYIRI, L. Change in the Rate of Deacylation of Benzylpenicillin and Phenylacetyl Glycine during Submerged Cultivation of Escherichia coli. Nature 214, 1347–1349 (1967). https://doi.org/10.1038/2141347a0
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DOI: https://doi.org/10.1038/2141347a0
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