Abstract
THIS communication describes a technique for localizing intestinal disaccharidases developed from that of Dahlqvist and Brun1. Intestinal biopsies were obtained by a multipurpose suction biopsy tube2 from four normal children and five patients with sucrose and isomaltose “intolerance”. Intestinal specimens from rats and guinea-pigs have also been investigated by the same procedure. The biopsies and animal tissues were rapidly frozen and stored at −25°. Sections from SJJL were cut in a cryostat, mounted on coverslips and fixed in cold acetone for 15 min.
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References
Dahlqvist, A., and Brun, A., J. Histochem. Cytochem., 10, 294 (1962).
Brandborg, L. L., Rubin, C. E., and Quinton, W. E., Gastroenterology, 37, 1 (1959).
Rosa, C. G., and Tsou, K. C., Nature, 192, 990 (1961).
Auricchio, S., Rubino, A., Prader, A., Rey, J., Jos, J., Frézal, J., and Davidson, M., J. Pediat., 66, 555 (1965).
Miller, D., and Crane, R. K., Biochim. Biophys. Acta, 52, 293 (1961).
Doell, R., Rosen, G., and Kretchmer, N., J. Pediat., 65, 1118 (1964).
Lojda, Z., Histochemie, 5, 339 (1965).
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JOS, J., FRÉZAL, J., REY, J. et al. Histochemical Localization of Intestinal Disaccharidases: Application to Peroral Biopsy Specimens. Nature 213, 516–518 (1967). https://doi.org/10.1038/213516a0
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DOI: https://doi.org/10.1038/213516a0
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