Abstract
PREVIOUS investigations have shown that 5-iodo-deoxyuridine (IUDR), an analogue of thymidine, is incorporated into DNA and within certain limitations can be used as an indicator of DNA synthesis (refs. 1–4, and work of Kreuger and others in preparation). Details of incorporation and elimination of iodo-deoxyuridine in various cells of mice may be found in a review4. According to the authors IUDR is a “convenient, sensitive, and specific tracer of DNA metabolism in vivo”. Incorporation of IUDR labelled with iodine-131 has been used as an indication of the rate of DNA synthesis at different stages of the developing sea urchin embryo5. The facility of detecting the γ-radiation of iodine-131 made it seem possible to use iodine-131 labelled IUDR to follow DNA synthesis in the same group of living cells without fixation, extraction, or digestion of the cells. This communication pursues the use of repetitive exposure to labelled IUDR of a single colony of sea urchin embryos as a method for the investigation of developmental changes in the rate of DNA synthesis in the living cell.
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References
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WHEELER, M., GREBE, S., HARDING, C. et al. Uptake of 5-lodo-deoxyuridine by a Single Colony of Developing Embryos of Arbacia punctulata. Nature 213, 514–515 (1967). https://doi.org/10.1038/213514a0
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DOI: https://doi.org/10.1038/213514a0
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