Abstract
THE difficulty of raising leopard frogs, Rana pipiens, to maturity in controlled conditions makes them impractical for many experiments1. Tadpoles have previously died soon after metamorphosis2, but we have now raised frogs to sexual maturity with a low mortality rate. Thus sibling adults can be used for experimental purposes, and using our methods (described here) it should be possible to carry out genetic studies over several generations and to develop partially inbred lines of these laboratory animals.
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References
Volpe, E. P., J. Heredity, 47, 79 (1956).
Anderson, S. C., and Volpe, E. P., Science, 127, 1048 (1958).
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CAIRNS, A., BOCK, J. & BOCK, F. Leopard Frogs raised in Partially Controlled Conditions. Nature 213, 191–193 (1967). https://doi.org/10.1038/213191a0
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DOI: https://doi.org/10.1038/213191a0
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