Abstract
MAMMALIAN germ cells have been utilized by several investigators for the investigation of the biochemical and physiological mechanisms of senescence in living cells1. Functionally and metabolically active bovine sperm have been successfully maintained and studied at room temperature for a period of 6 days or more2. The results of these studies led to the present work in which two chemically defined media were used to maintain large numbers of vigorously motile rabbit sperm. The two media tested here were ‘Coconut Milk Extender’ (CME)3 and Norman-Johnson solution No. 2 (N-J-2)4, a chemically defined medium. Over a 6 day period of storage at room temperature (24° ± 2° C), N-J-2 proved to be the more satisfactory medium for supporting survival and activity of the sperm.
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GULYAS, B. In vitro Maintenance of Rabbit Sperm at Room Temperature. Nature 211, 987 (1966). https://doi.org/10.1038/211987a0
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DOI: https://doi.org/10.1038/211987a0
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