Abstract
PREVIOUS work on tissue cultures of the wild carrot has shown that the embryogenesis which occurs in such cultures is not fundamentally different from the same process in the ovule1,2. It has also been shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) which is higher than 0.1 mg/1. (ref. 3). Lowering the 2,4-D concentration permits histological differentiation and polarized growth to occur. It is thus possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,4-D concentration. While this technique has provided an experimental system in which the simultaneous development of numerous embryos can be examined, it has provided no information on the morphogenetic factors which initially cause cultured cells to behave as zygotes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Halperin, W., and Wetherell, D. F., Amer. J. Bot., 51, 274 (1964).
Halperin, W., and Wetherell, D. F., Science (in the press).
Halperin, W., Science, 146, 408 (1964).
Raghavan, V., and Torrey, J. G., Amer. J. Bot., 51, 264 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HALPERIN, W., WETHERELL, D. Ammonium Requirement for Embryogenesis in vitro. Nature 205, 519–520 (1965). https://doi.org/10.1038/205519a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/205519a0
This article is cited by
-
The source, level, and balance of nitrogen during the somatic embryogenesis process drive cellular differentiation
Planta (2022)
-
Advances in coffee tissue culture and its practical applications
In Vitro Cellular & Developmental Biology - Plant (2007)
-
Regulating plant tissue growth by mineral nutrition
In Vitro Cellular & Developmental Biology - Plant (2007)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.