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Photographic Detection of Bands in Gel-diffusion Precipitin Tests with Viruses

Abstract

BANDS of precipitation in gel-diffusion precipitin tests with viruses are usually recorded by photographing the bands with a suitably arranged light source, and a refinement of this method was described by Klontz et al.1. Markham and Smith2 showed that the position of ultraviolet light-absorbing fractions separated by paper chromatography could be recorded by photographing the paper in ultraviolet light. Weintraub et al.3 used an analogous method to record the position of viruses which were fractionated by agar-gel electrophoresis. This communication describes a similar method for recording the position of bands produced in gel-diffusion precipitin reactions between viruses and antibodies.

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References

  1. Klontz, G. W., Ridgway, G. J., and Wilson, G. P., J. Biol., Photographic Assoc., 28, 11 (1960).

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  2. Markham, R., and Smith, J. D., Nature, 163, 250 (1949).

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  3. Weintraub, M., Ragetli, H. W. J., and Townsley, P. M., Virology, 17, 196 (1962).

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  4. Markham, R., in Modern Methods of Plant Analysis, edit. by Paech, K., and Tracey, M. V., 4, 246 (Springer, Berlin, 1955).

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THOMSON, A. Photographic Detection of Bands in Gel-diffusion Precipitin Tests with Viruses. Nature 201, 422–423 (1964). https://doi.org/10.1038/201422a0

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