Abstract
DISCOVERY of an in vitro action of a hormone is of intrinsic value in determining the detailed mechanism of its action—a situation well illustrated in recent years in the case of insulin. Pituitary growth hormone is known to have an action in vitro on isolated muscle preparations—to stimulate uptake of glucose1,2, incorporation of amino-acids into protein3,4 and accumulation of a non-utilizable amino-acid5 by diaphragm from the hypophysectomized rat, and to influence the metabolism of adipose tissue6. Whether these in vitro actions of the hormone play any part in bringing about its growth-promoting action has not been clarified2,4,5,7–9. The purpose of this communication is to show that by column chromatography ox growth hormone can be resolved into three fractions which, though all retaining potency in vitro, possess markedly differing growth-promoting activities.
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MANCHESTER, K., WALLIS, M. Comparison of in vivo and in vitro Actions of Various Ox Pituitary Growth Hormone Fractions. Nature 200, 888–889 (1963). https://doi.org/10.1038/200888a0
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DOI: https://doi.org/10.1038/200888a0
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