Abstract
WORK from this laboratory has shown that transglutaminase catalyses the hydrolysis of the amide group of protein-bound glutamine, and its exchange with primary amines1. In addition, preliminary evidence indicated that the ε-amino group of protein-bound lysine may act as a replacing amine2. Therefore, by the action of transglutaminase, various proteins can be modified and the new protein species may have properties unlike those of the original protein; for example, proteins may acquire novel antigenic properties as shown by proteins in which the ε-amino group of lysine had been modified by substitution with polyamino-acids by chemical means3,4. Modifications produced enzymatically by the linkage of two proteins to each other through the γ-carboxyl group of protein-bound glutamic acid and the ε-amino group of protein-bound lysine are of particular interest.
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References
Clarke, D. D., Mycek, M. J., Neidle, A., and Waelsch, H., Arch. Biochem. Biophys., 79, 338 (1959).
Neidle, A., Mycek, M. J., Clarke, D. D., and Waelsch, H., Arch. Biochem. Biophys., 77, 227 (1958).
Buchanan-Davidson, D. J., Dellert, E. E., Kornguth, S. E., and Stahmann, M. A., J. Immunol., 83, 543 (1959).
Van Kley, H., Kornguth, S. E., and Stahmann, M. A., J. Amer. Chem. Soc., 81, 4370 (1959).
Neidle, A., and Acs, G., Fed. Proc., 20, 234 (1961).
Kies, M. W., and Alvord, jun., E. C., ‘Allergic’ Encephalomyelitis, edit., by Kies, M. W., and Alvord, jun., E. C., 293 (Charles C. Thomas. Springfield, Ill., 1959).
Waelsch, H., and Mycek, M. J., Methods in Enzymology, edit. by Colowick, S. P., and Kaplan, N. O., 5, 833 (Academic Press, New York, 1962).
Wajda, I., Ginsburg, M., and Waelsch, H., Nature, 191, 1204 (1961).
Lee, J. M., and Olitsky, P. K., Proc. Soc. Exp. Biol. Med., 89, 263 (1955).
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KORNGUTH, S., WAELSCH, H. Protein Modifications catalysed by Transglutaminase. Nature 198, 188–189 (1963). https://doi.org/10.1038/198188a0
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DOI: https://doi.org/10.1038/198188a0
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