Abstract
WORK from this laboratory has shown that transglutaminase catalyses the hydrolysis of the amide group of protein-bound glutamine, and its exchange with primary amines1. In addition, preliminary evidence indicated that the ε-amino group of protein-bound lysine may act as a replacing amine2. Therefore, by the action of transglutaminase, various proteins can be modified and the new protein species may have properties unlike those of the original protein; for example, proteins may acquire novel antigenic properties as shown by proteins in which the ε-amino group of lysine had been modified by substitution with polyamino-acids by chemical means3,4. Modifications produced enzymatically by the linkage of two proteins to each other through the γ-carboxyl group of protein-bound glutamic acid and the ε-amino group of protein-bound lysine are of particular interest.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Clarke, D. D., Mycek, M. J., Neidle, A., and Waelsch, H., Arch. Biochem. Biophys., 79, 338 (1959).
Neidle, A., Mycek, M. J., Clarke, D. D., and Waelsch, H., Arch. Biochem. Biophys., 77, 227 (1958).
Buchanan-Davidson, D. J., Dellert, E. E., Kornguth, S. E., and Stahmann, M. A., J. Immunol., 83, 543 (1959).
Van Kley, H., Kornguth, S. E., and Stahmann, M. A., J. Amer. Chem. Soc., 81, 4370 (1959).
Neidle, A., and Acs, G., Fed. Proc., 20, 234 (1961).
Kies, M. W., and Alvord, jun., E. C., ‘Allergic’ Encephalomyelitis, edit., by Kies, M. W., and Alvord, jun., E. C., 293 (Charles C. Thomas. Springfield, Ill., 1959).
Waelsch, H., and Mycek, M. J., Methods in Enzymology, edit. by Colowick, S. P., and Kaplan, N. O., 5, 833 (Academic Press, New York, 1962).
Wajda, I., Ginsburg, M., and Waelsch, H., Nature, 191, 1204 (1961).
Lee, J. M., and Olitsky, P. K., Proc. Soc. Exp. Biol. Med., 89, 263 (1955).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KORNGUTH, S., WAELSCH, H. Protein Modifications catalysed by Transglutaminase. Nature 198, 188–189 (1963). https://doi.org/10.1038/198188a0
Issue Date:
DOI: https://doi.org/10.1038/198188a0
This article is cited by
-
Inhibition of Transglutaminase Exacerbates Polyglutamine-Induced Neurotoxicity by Increasing the Aggregation of Mutant Ataxin-3 in an SCA3 Drosophila Model
Neurotoxicity Research (2015)
-
Transglutaminases
Molecular and Cellular Biochemistry (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
