Abstract
WE have been looking for a quick and relatively cheap method of screening dehydrogenase isozymes, after electrophoresis. For this purpose, we chose agar gel as a support. We recognize that there is a greater diffusion on agar gel than on starch gel, and also that there is some difficulty in slicing the gel before detecting the isoenzymes. Nevertheless, we prefer agar gel because: (1) it allows a relatively quick electrophoretic partition; (2) it is transparent when dry; (3) it allows the use of the Grabar and Williams technique of immunoelectrophoresis1.
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Grabar, P., and Williams, C. A., Biochim. Biophys. Acta, 17, 65 (1955).
Vesell, E. S., Ann. N.Y. Acad. Sci., 94, 877 (1961).
Markert, C. L., and Appella, E., Ann. N.Y. Acad. Sci., 94, 678 (1961).
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BROUN, G., AVRAMEAS, S. Visualization of Different Diphospho- and Triphospho-pyridine Nucleotide Dehydrogenase Isozymes on an Agar-gel Plate. Nature 197, 1208 (1963). https://doi.org/10.1038/1971208a0
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DOI: https://doi.org/10.1038/1971208a0
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