N-terminal Amino-Acids of Human Haptoglobins

Abstract

HAPTOGLOBINS have been prepared from unhæmolysed citrated human plasma, pooled for haptoglobin type, by a modification of the method of Connell and Shaw¹. After dialysis against acetate buffer (0.01 M pH 4.7) the plasma was applied to a DEAE cellulose column at 3° C. previously equilibrated with the acetate buffer. Most of the non-haptoglobin material was removed by washing the column with the same acetate buffer containing 0.01 M sodium chloride. The column was then treated with acetate buffer containing 0.04 M sodium chloride adjusted to pH 4.7, and the effluent collected. After adjustment of the pH to 7.0 ± 0.5, the volume was reduced on a rotary film evaporator, dialysed against water and the resulting solution lyophilized. The product was finally fractionated with ammonium sulphate dialysed and lyophilized.