Abstract
THE term thyroxine-binding globulin is often used to denote a serum protein fraction with a mobility intermediate between those of α1 - and α2-globulins in paper or moving-boundary electrophoresis in veronal buffer, pH 8.6, ionic strength 0.05–0.10 (ref. 1). However, with the introduction of supporting media such as agar and starch gels and the use of different buffers, ionic strength or pH for paper electrophoresis, binding of thyroid hormones has also been detected in the ‘prealbumin’ zone2. Although the proteins of the ‘prealbumin’ zone obtained under different conditions are not likely to be identical, a strong thyroxine-binding property was detected in human serum prealbumin rich in tryptophan3, as obtained from Prof. Schultze4. From chemical and immunological observations I concluded that the tryptophanrich prealbumin, present as a complex in the α-globulin fraction, might be responsible for much of thyroxine-binding in human serum5. Since then, an α-globulin-type of thyroxine-binding protein has been obtained in this laboratory (unpublished results); the nature of this protein does not support the hypothesis that the tryptophan-rich prealbumin is involved in binding thyroxine. One possible explanation for this discrepancy is that Schultze's prealbumin preparation contained traces (1–2 per cent) of thyroxine-binding globulin which were responsible for the production of antibodies to it present in antisera against this prealbumin. In fact, multiple precipitation lines were observed in immunological studies using rabbit anti-human prealbumin serum5. The experiments to be described support this explanation and throw further light on the nature of the thyroxine-binding globulin in human serum.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Robbins, J., and Rall, J. E., Rec. Progr. Hormone Res., 13, 161 (1957). Pitt-Rivers, R., and Tata, J. R., The Thyroid Hormones (Pergamon Press, London, 1959). Tata, J. R., Brit. Med. Bull., 16, 142 (1960).
Rich, C., and Bearn, A. G., Endocrinol., 62, 687 (1958). Ingbar, S. H., ibid., 63, 256 (1958). Tata, J. R., Brit. Med. Bull., 16, 142 (1960). Myant, N. B., and Osorio, C., J. Physiol. (in the press).
Tata, J. R., Biochem. J., 72, 222 (1959).
Schultze, H. E., Schönenberger, M., and Schwick, G., Biochem. Z., 328, 267 (1956).
Tata, J. R., Nature, 183, 877 (1959).
Ouchterlony, O., Acta Path. Microbiol. Scand., 25, 186 (1948).
Allison, A. C., and Humphrey, J. H., Immunol., 3, 95 (1960).
Schultze, H. E., Göllner, I., Heide, K., Schönenberger, M., and Schwick, G., Z. Naturf., 10, b, 463 (1955). Schmid, K., Bencze, W. L., Nussbaumer, T., and Wehrmüller, J. O., J. Biol. Chem., 234, 529 (1959).
Robbins, J., Petermann, M. L., and Rall, J. E., J. Biol. Chem., 212, 403 (1955). Petermann, M. L., Robbins, J., and Hamilton, M. G., J. Biol. Chem., 208, 369 (1954).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TATA, J. Nature of Thyroxine-binding Globulin in Human Serum. Nature 189, 573–574 (1961). https://doi.org/10.1038/189573a0
Issue Date:
DOI: https://doi.org/10.1038/189573a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.