Abstract
NITRATE reduction in Neurospora is usually considered to be an assimilatory process. Nitrate reductase is a flavoprotein containing molybdenum, and the sequence of electron transfer has been determined as follows : 
where TPNH is reduced triphosphopyridine nucleotide and FAD is flavin-adenine-dinucleotide. It was shown that a deficiency of iron in the felts grown under aerobic conditions did not depress enzyme activity2,3. In this communication we report that Neurospora grown at low oxygen tensions dissimilates nitrate, and under these conditions nitrate reductase is dependent on iron as well as molybdenum for maximum activity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Nicholas, D. J. D., and Nason, A., J. Biol. Chem., 211, 183 (1954).
Nicholas, D. J. D., Nason, A., and McElroy, W. D., J. Biol. Chem., 207, 341 (1954).
Nicholas, D. J. D., J. Sci. Food Agric., 8, 515 (1957).
Nicholas, D. J. D., and Fielding, A. H., J. Hort. Sci., 26, 125 (1951).
Wosilait, W. D., and Nason, A., J. Biol. Chem., 206, 255 (1954).
Lowry, O. H., Rosenbrough, W. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Fewson, C. A., and Nicholas, D. J. D., Biochem. J., 77, 3 (1960).
Verhoeven, W., and Takeda, Y., in “Inorganic Nitrogen Metabolism”, edit. by McElroy and Glass, 159 (Johns Hopkins Univ. Press, Baltimore, 1956).
Sato, R., in “Inorganic Nitrogen Metabolism”, edit. by McElroy and Glass, 163 (Johns Hopkins Univ. Press, Baltimore, 1956.)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WALKER, G., NICHOLAS, D. An Iron Requirement for a Dissimilatory Nitrate Reductase in Neurospora crassa . Nature 189, 141–142 (1961). https://doi.org/10.1038/189141a0
Issue Date:
DOI: https://doi.org/10.1038/189141a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
