Abstract
IN the course of studies on the maturation of red blood cells we confirmed the report of Malkin and Denstedt1 that reticulocytes show a tenfold higher activity of inorganic pyrophosphatase as compared with erythrocytes. Pyrophosphatase appears to contain a functional SH— group. Traces of cupric ions, para-chloromercury benzoate and iodo-acetate cause an inhibition which is reversible by cysteine2,3. To test the assumption that the lability of the SH— group may be connected with the decrease of pyrophosphatase activity during maturation, the effect of conditions which change the oxidation status of glutathione on the activity of pyrophosphatase was studied.
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RAPOPORT, S., SCHEUCH, D. Glutathione Stability and Pyrophosphatase Activity in Reticulocytes; Direct Evidence for the Importance of Glutathione for the Enzyme Status in Intact Cells. Nature 186, 967–968 (1960). https://doi.org/10.1038/186967a0
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DOI: https://doi.org/10.1038/186967a0
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