Abstract
A VERSATILE method of amino-acid determination is that in which amino-acid solutions are incubated with cupric orthophosphate in suspension. The resulting solution contains copper in proportion to the total molarity of amino-acids and the copper is determined by any suitable method. The methods used have included iodometric titration1 and the use of cupric phosphate labelled with copper-64 followed by the determination of the activity in the supernatant2. The latter method is of particular use for the continuous determination of amino-acids in chromatographic eluates.
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VINCENT, W. Colorimetric Determination of Amino-acid Concentration. Nature 185, 530 (1960). https://doi.org/10.1038/185530a0
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DOI: https://doi.org/10.1038/185530a0
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