Abstract
ELECTROPHORESIS on paper of serum proteins has enabled us to separate small quantities into albumin, and α-, β- and γ-globulin. By using a comparatively thick filter paper (MunktellNr. 20, Grycksbo, Sweden), 58 cm. × 7 cm., we can separate up to 30 mgm. of protein within 24 hr., with 5.5 V./cm. and 16–18 m.amp. applied in a closed atmosphere. As buffer system veronal/veronal sodium is used, of 0.06 ionic strength and pH 8.9. From the two paper strips used simultaneously, only one is stained afterwards with bromphenol blue; this strip serves as a guide for cutting off the fractions from the still wet, unstained strip.
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References
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Wunderly, Ch., and Wuhrmann, F., “Liver Disease”, CIBA Foundation Symp., London, 1951, 11f.; Schweiz. Med. Wschr., 81, 1102 (1951).
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WUNDERLY, C. Lyophilic Properties of Isolated Serum-Protein Fractions. Nature 169, 932–933 (1952). https://doi.org/10.1038/169932b0
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DOI: https://doi.org/10.1038/169932b0
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