Abstract
THE fickle nature of the homogeneity test as applied to partition chromatography has been well illustrated in a recent article on double zoning by Dr. Lester Smith1. The singularity of the chromatographic zone, though sound enough in theory, is no doubt taken too much for granted in applications in which conditions are not so simple as theory assumes. It seems relevant, now, to place on record conditions which I have found conducive to double zoning of a similar type on adsorption chromatograms, and which were too briefly mentioned at a symposium in 1946 2.
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References
Lester Smith, E., Nature, 169, 60 (1952).
Analyst, 71, 266 (1946).
Ovenston, T. C. J., J. Soc. Chem. Indust., 68, 54 (1949).
Schroeder, W. A., Ann. N.Y. Acad. Sci., 49, 205, 211 (1948).
Levy, W. J., and Campbell, N., J. Chem. Soc., 1445 (1939).
Parker, C. A., J. Soc. Chem. Indust., 67, 434 (1948).
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OVENSTON, T. Double Zoning and the Homogeneity Test in Column Chromatography. Nature 169, 924–925 (1952). https://doi.org/10.1038/169924a0
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DOI: https://doi.org/10.1038/169924a0
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