Abstract
TRANSAMINASE reactions are known to occur in practically all animal tissues, especially in heart muscle, brain, kidney, liver and testes. Reactions of this type were first suggested by Needham1, who noted that when glutamic acid was added to minced pigeon-breast muscle it disappeared without a parallel formation of ammonia. Four or five different transaminase systems have so far been thoroughly investigated by various workers2; but the methods employed for the study of these reactions have required manometric equipment3 and intensive analytical procedures4. The present communication deals with the application of paper chromatography technique to the separation of 2 : 4 dinitrophenyl-hydrazones of the keto-acids in the reaction mixture, since it has proved in our hands a satisfactory tool for the study of transaminase reactions. The reaction studied was: L-Aspartate + α-Ketoglutarate = Oxalacetate + L-Glutamate.
Similar content being viewed by others
Article PDF
References
Needham, D. M., Biochem. J., 24, 208 (1930).
Cohen, P. P., J. Biol. Chem., 136, 565 (1940). Cohen, P. P., and Hekhuis, G. L., J. Biol. Chem., 140, 711 (1941). Herbst, R. M., “Advance in Enzymol.”, 4, 75 (1944).
Ames, S. R., and Elvehjem, C. A., J. Biol. Chem., 166, 81 (1946). Ames, S. R., Sarma, P. S., and Elvehjem, C. A., J. Biol. Chem., 167, 135 (1947).
Cohen, P. P., Biochem. J., 33, 1478 (1939).
Tonhazy, N. E., White, N. G., and Umbreit, W. W., Arch. Biochem., 28, 36 (1950).
Cavallini, D., Frontali, N., and Toschi, G., Nature, 163, 568 (1949).
Bueding, E., and Wortis, H., J. Biol. Chem., 133, 585 (1940).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TULPULE, P., PATWARDHAN, V. Application of Paper Chromatography to the Study of the Transaminase System. Nature 169, 671 (1952). https://doi.org/10.1038/169671a0
Issue Date:
DOI: https://doi.org/10.1038/169671a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.